HLA-A*02:01 CLGGLLTMV peptide complex Tetramer
Categories: HLA-A peptide complexesMHC peptide complexes$510.00 – $2,030.00
Description
Accession
A0A140T913
Source
DNA sequence encoding the Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and CLGGLLTMV peptide.This complex includes a C terminal C-His-Avi tag and was expressed in HEK cells.
Molecular weight
Recombinant HLA-A*02:01 CLGGLLTMV peptide complex is a glycosylated protein. The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant HLA-A*02:01 CLGGLLTMV peptide complex was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human LMP2 (HLA-A*02:01) Tetramer Protein ELISA data
- Dose response curve for Human LMP2 (HLA-A*02:01) Tetramer Protein was determined by ELISA.
Human LMP2 (HLA-A*02:01) Tetramer Protein SEC-HPLC
- The purity of Human LMP2 (HLA-A*02:01) Tetramer Proteinwas greater than 95% as determined by SEC-HPLC.
Human LMP2 (HLA-A*02:01) Tetramer Protein Tris-Bis PAGE
- Human LMP2 (HLA-A*02:01) Tetramer Protein PAGE under reduced condition. The purity is greater than 95%.
