Staphylococcal Protein A (SPA) Recombinant

/Staphylococcal Protein A (SPA) Recombinant

Staphylococcal Protein A (SPA) Recombinant


SKU: RKP02976 Tags: , , ,

accession P02976

Source Optimized DNA sequence encoding Staphylococcus Aureus IgG Binding domain repeat was expressed in Escherichia Coli.
Molecular weight Recombinant Protein A, is a monomer protein consisting of 300 amino acid residue subunits. The molecule has a calculated molecular mass of approximately 34 kDa.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity Activity was determined by a binding capacity assay to human IgG, and was found to be not less than 95%.
Applications Elisa,WB,Affinity purification of monoclonals and polyclonals,Immunoprecipitation.
Presentation Recombinant Protein A was lyophilized from a 0.2 μm filtered solution.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Molecular function IgG-binding-protein