SARS COV2 Spike protein S Trimer Recombinant
$350.00 – $1,300.00
| accession | NCOVS |
| Source | DNA sequence encoding the COVID-19 Spike S protein extracellular domain,[16-1188]R683A (accession # QHD43416.1) fused to a a T4 Folsom trimerzation domain and a C-terminal His AVI tag was expressed in HEK Cells. |
| Molecular weight | Recombinant 2019-nCoV Spike S trimer protein is a protein consisting of the S1+S2 subunits amino acid residues. The protein has a theoretical MW of 140kDa,however due to glycosylation migrates as an approximately 200 kDa protein on reduced SDS-PAGE. |
| Activity | The activity was tested by binding Human ACE2 in functional ELISA assay (see figure below). |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | Recombinant nCoV-2019 Spike S trimer protein was lyophilized from 0.2 µm filtered PBS pH7.4. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. |
| Usage | This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
Methods
SARS COV2 S trimer ACE functional binding ELISA assay
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S trimer protein PAGE
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SARS COV-2 Spike S trimer HPLC
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