SARS COV2 Spike protein S Recombinant-Fc

///SARS COV2 Spike protein S Recombinant-Fc

SARS COV2 Spike protein S Recombinant-Fc

$350.00$1,200.00


accession NCOVS


Source DNA sequence encoding the COVID-19 Spike S protein extracellular domain,[14-1211] accession #QHD43416.1 including a C-terminal Fc AVI tag was expressed in HEK Cells. (The furin protease cleavage site was mutated at position 683)
Molecular weight Recombinant 2019-nCoV Spike S protein is a protein consisting of the S1+S2 subunits with a calculated MW of 160kDa ,due to glycosylation migrates as an approximately 220 kDa protein on SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant nCoV-2019 Spike S protein was lyophilized from 0.2 µm filtered PBS pH 7.4
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.



Methods

SARS COV2 S-Fc protein PAGE

  • 1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.

SARS COV-2 Spike S-Fc HPLC

  • The purity of 2019-nCoV Spike S is greater than 95% as determined by SEC-HPLC.