Recombinant Human FAS Ligand

Recombinant Human FAS Ligand

$70.00$4,700.00


accession P48023


Source Optimized DNA sequence encoding extracellular TNF domain of Human FAS Ligand mature chain was expressed in Chinese Hamster Ovary Cells.
Molecular weight Recombinant human FAS Ligand is a monomer protein consisting of 146 amino acid residue subunits, and migrates as an approximately 18 kDa protein under reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC.
Biological Activity The ED(50) was determined by the ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linkingand was found to be in the range of.0 ng/ml.

Protein Sequence MQQPFNYPYP QIYWVDSSAS SPWAPPGTVL PCPTSVPRRP GQRRPPPPPP PPPLPPPPPP PPLPPLPLPP LKKRGNHSTG LCLLVMFFMV LVALVGLGLG MFQLFHLQKE LAELRESTSQ MHTASSLEKQ IGHPSPPPEK KELRKVAHLT GKSNSRSMPL EWEDTYGIVL LSGVKYKKGG LVINETGLYF VYSKVYFRGQ SCNNLPLSHK VYMRNSKYPQ DLVMMEGKMM SYCTTGQMWA RSSYLGAVFN LTSADHLYVN VSELSLVNFE ESQTFFGLYK L
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation FAS Ligand was lyophilized from a.2 μm filtered solution in.5% glycine,.5% sucrose,.01% Tween80, mM Glutamic acid, pH.5.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor O95150 TNF15_HUMAN
Interactor P16333
Interactor O43557 TNF14_HUMAN
Interactor P01730 CD4_HUMAN
Interactor P25445 TNR6_HUMAN
Interactor P48023 TNFL6_HUMAN
Interactor Q13158
Interactor Q14790 CASP8_HUMAN
Interactor Q13158
Biological Process Transcription
Molecular function Cytokine
Molecular function Repressor

Methods

Different effects of cell release by trypsin and NECDS on subsequent initiation of anoikis and on antibody accessibility to the cell surface anoikis-initiating molecules in MUC1-positive and -negative cells.

Recombinant Fas-L
  • (b–d) Representative flow cytometry plots show antibody access to cell surface MUC1 (b), E-cadherin, Integrinβ1, CD44 (c, d) and recombinant Fas-L access to cell surface Fas (f) in HCA1.7+/− cells released by trypsin or NECDS.