Rat Vascular Endothelial Growth Factor 164AA Recombinant
$70.00 – $3,500.00
| accession | P16612 |
| Source | Optimized DNA sequence encoding rat VEGF mature chain was expressed in Escherichia Coli. |
| Molecular weight | Native human rat VEGF is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately kDa. Recombinant rat VEGF is a disulfide-linked homodimeric protein consisting of two amino acid residue subunits, and migrates as an approximately kDa protein under non-reducing conditions and as kDa under reducing conditions in SDS-PAGE. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | The ED(50) was determined by the dose-dependent proliferation of Human umbilical vein endothelial cells (HUVEC)was found to be in the less than.0 ng/ml. |
| Protein Sequence | MNFLLSWVHW TLALLLYLHH AKWSQAAPTT EGEQKAHEVV KFMDVYQRSY CRPIETLVDI FQEYPDEIEY IFKPSCVPLM RCAGCCNDEA LECVPTSESN VTMQIMRIKP HQSQHIGEMS FLQHSRCECR PKKDRTKPEN HCEPCS ERRK HLFVQDPQTC KCSCKNTDSR CKARQL ELNE RTCRCD KPRR |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Recombinant rat VEGF was lyophilized from a 0.2 μm filtered PBS solution. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Biological Process | Angiogenesis |
| Biological Process | Differentiation |
| Molecular function | Developmental-protein |
| Molecular function | Growth-factor |
| Molecular function | Heparin-binding |
Methods
Culture of sorted BM Sca-1+/Lin− cells for cardiomyogenic and vasculogenic differentiation and quantitative RT-PCR
|
Media preparation
|
