Rat Stem Cell Factor Recombinant

/, PDGF family, Recombinant Rat Cytokines/Rat Stem Cell Factor Recombinant

Rat Stem Cell Factor Recombinant

$70.00$2,700.00


accession P21581


Source Optimized DNA sequence encoding Rat SCF mature chain was expressed in Escherichia Coli.
Molecular weight Native Rat Stem Cell Factoris generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately kDa. Recombinant SCF is a disulfide-linked monomer protein consisting of amino acid residue subunits, andmigrates as an approximately18 kDa protein under non-reducingand reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity The ED(50) was determined by the dose-dependent stimulation of theproliferation of human TF-1 cells is ≤.0 ng/ml, corresponding to a specific activity of ≥ x units/mg
Protein Sequence MKKTQTWIIT CIYLQLLLFN PLVKTQEICR NPVTDNVKDI TKLVANLPND YMITLNYVAG MDVLPSHCWL RDMVTHLSVS LTTLLDKFSN ISEGLSNYSI IDKLGKIVDD LVACMEENAP KNVKESLKKP ETRNFTPEEF FSIFNRSIDA FKDFMVASDT SDCVLSSTLG PEKDSRVSVT KPFMLPPVA A SSLRNDSSSS NRKAAKSPED PGLQWTAMAL PALISLVIGF AFGALYWKKK QSSLTRAVEN IQINEEDNEI SMLQQKEREF QEV
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant Rat Stemcell factor was lyophilized from a.2 μm filteredNaHCO3 solution.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Biological Process Cell-adhesion
Molecular function Growth-factor

Methods

Pathological examination of livers and kidneys after SCF+G-CSF treatment during chronic stroke in aged mice.

recombinant rat SCF
  • S+G 200/50: SCF (200 μg/kg) in combination with G-CSF (50 μg/kg).

Effect of stem cell factor (SCF) on cardiac stem cell (CSC) migration in vitro.

50 ng/ml recombinant rat SCF
  • Representative images of migrated CSCs treated with varying concentrations of SCF by Transwell-based migration assays.