Rat Stem Cell Factor Recombinant
$70.00 – $2,700.00
| accession | P21581 |
| Source | Optimized DNA sequence encoding Rat SCF mature chain was expressed in Escherichia Coli. |
| Molecular weight | Native Rat Stem Cell Factor is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 18 kDa. Recombinant SCF is a monomer protein consisting of 165 amino acid residue subunits, and migrates as an approximately 18 kDa protein under reducing conditions in SDS-PAGE. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | The ED(50) was determined by the dose-dependent stimulation of theproliferation of human TF-1 cells is ≤.0 ng/ml, corresponding to a specific activity of ≥ x units/mg |
| Protein Sequence | MKKTQTWIIT CIYLQLLLFN PLVKTQEICR NPVTDNVKDI TKLVANLPND YMITLNYVAG MDVLPSHCWL RDMVTHLSVS LTTLLDKFSN ISEGLSNYSI IDKLGKIVDD LVACMEENAP KNVKESLKKP ETRNFTPEEF FSIFNRSIDA FKDFMVASDT SDCVLSSTLG PEKDSRVSVT KPFMLPPVA A SSLRNDSSSS NRKAAKSPED PGLQWTAMAL PALISLVIGF AFGALYWKKK QSSLTRAVEN IQINEEDNEI SMLQQKEREF QEV |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Recombinant Rat Stemcell factor was lyophilized from a.2 μm filteredNaHCO3 solution. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Biological Process | Cell-adhesion |
| Molecular function | Growth-factor |
Methods
Pathological examination of livers and kidneys after SCF+G-CSF treatment during chronic stroke in aged mice.
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Effect of stem cell factor (SCF) on cardiac stem cell (CSC) migration in vitro.
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