/, PDGF family, Recombinant Rat Cytokines/Rat Stem Cell Factor Recombinant

Rat Stem Cell Factor Recombinant

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$70.00$2,700.00

SKU: RKP21581 Tags: , , , , , , , ,

Description

Accession
P21581
Source
Optimized DNA sequence encoding Rat SCF mature chain was expressed in Escherichia Coli.
Molecular weight
Native Rat Stem Cell Factor is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 18 kDa. Recombinant SCF is a monomer protein consisting of 165 amino acid residue subunits, and migrates as an approximately 18 kDa protein under reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the dose-dependent stimulation of theproliferation of human TF-1 cells is ≤.0 ng/ml, corresponding to a specific activity of ≥ x units/mg
Protein Sequence
MKKTQTWIIT CIYLQLLLFN PLVKTQEICR NPVTDNVKDI TKLVANLPND YMITLNYVAG MDVLPSHCWL RDMVTHLSVS LTTLLDKFSN ISEGLSNYSI IDKLGKIVDD LVACMEENAP KNVKESLKKP ETRNFTPEEF FSIFNRSIDA FKDFMVASDT SDCVLSSTLG PEKDSRVSVT KPFMLPPVA A SSLRNDSSSS NRKAAKSPED PGLQWTAMAL PALISLVIGF AFGALYWKKK QSSLTRAVEN IQINEEDNEI SMLQQKEREF QEV
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant Rat Stemcell factor was lyophilized from a.2 μm filteredNaHCO3 solution.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function

Methods

Pathological examination of livers and kidneys after SCF+G-CSF treatment during chronic stroke in aged mice.

  • S+G 200/50: SCF (200 μg/kg) in combination with G-CSF (50 μg/kg).

Effect of stem cell factor (SCF) on cardiac stem cell (CSC) migration in vitro.

  • Representative images of migrated CSCs treated with varying concentrations of SCF by Transwell-based migration assays.
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