-
Oligodendrocyte. Cells were seeded at a concentration of 5,000 cells/cm2 on tissue culture plastic plates and coverslips and cultured in high glucose DMEM supplemented with 1% Penicillin/Streptomycin , 2 mmol/l L-Glutamine , 1X N1 supplement , 1 µg/ml biotin , 5 ng/ml bFGF , 1 ng/ml PDGF , and 30% B104-conditioned media for 1 day.
- On the second day, CG4 rat oligodendrocyte progenitor cells were added in a co-culture setting to promote differentiation, using co-culture membrane inserts, and the media were changed every 2 days.
- The cells were allowed to differentiate for 5 days and were then fixed and stored in PBS for immunofluorescence.
- Cells were subsequently assessed for expression of the oligodendrocyte markers O2 and NG2.
|
Role of IL-8, PDGF-AB/BB and MMP9 molecules from conditioned media in migration, proliferation and ability of tubule formation of UCB ECFC derived cells.
-
Control medium with recombinant (rec) IL-8 or PDGF-AB/BB was also included.
|
Preparation of SLCs
-
After being subcultured at a concentration of 1 × 106 cells/cm2, BM-MSCs were incubated in αMEM containing 1 mM BME without serum for 24 h. The culture medium was then replaced with αMEM containing 10% FBS and 35 ng/ml at-RA .
- After three days, the cells were finally transferred to inducer medium containing αMEM, 10% FBS and trophic factors of 5 μM FSK , 10 ng/ml bFGF , 5 ng/ml PGF , and 200 ng/ml HG .
- The cells were cultured for 10 days [
|
SO2 derivatives suppressed VSMCs proliferation by inhibiting Erk/MAPK pathway.
-
Cells in coverslips were starved for 24 h and then pretreated with or without Na2SO3/NaHSO3 at 15 μmol/l for 30 min, as well as with PDGF-BB at 50 ng/ml treatment for 24 h for immunofluorescence assay of BrdU incorporation.
|
