rat MCP-1(CCL2) Recombinant
$70.00 – $2,700.00
| accession | P14844 |
| Source | Optimized DNA sequence encoding rat MCP-1(CCL2) mature chain was expressed in Escherichia Coli. |
| Molecular weight | rat MCP-1, generated by the proteolytic removal of the signal peptide and propeptide and has a calculated molecular mass of approximately 14 kDa. Recombinant MCP-1 is a monomeric protein consisting of 125 amino acid residue subunits and migrates as an approximately 14 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. |
| Purity | >98%, as determined by SDS-PAGE and HPLC |
| Biological Activity | Determined by its ability to chemoattract Balb/C mouse spleen MNCs using a concentration range of 1.0-20.0 ng/ml. |
| Sequence | QPDAVNAPLT CCYSFTGKMI PMSRLENYKR ITSSRCPKEA VVFVTKLKRE ICADPNKEWV QKYIRKLDQN QVRSETTVFY KIASTLRTSA PLNVNLTHKS EANASTLFST TTSSTSVEVT SMTEN |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | MCP-1 was lyophilized from PBS pH 7.4. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | This cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Biological Process | Chemotaxis |
| Biological Process | Inflammatory-response |
| Molecular function | Cytokine |
Methods
Bromodeoxyuridine labelling assay
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