Rat Leptin Recombinant
$70.00 – $450.00
| accession | P50596 |
| Source | Optimized DNA sequence encodingRatLeptin mature chain was expressed in Escherichia Coli. |
| Molecular weight | Rat Leptin is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately1 16 kDa. Recombinant Leptin is a monomer protein consisting of 147 amino acid residue subunits, and migrates as an approximately 16 kDa protein under non-reducing and reducing conditions in SDS-PAGE. |
| Purity | >98%, as determined by SDS-PAGE and HPLC |
| Biological Activity | Activitywas determined by the dose-dependent proliferation ofBAF/3 cells. |
| Protein Sequence | MCWRPLCRFL WLWSYLSYVQ AVPIHKVQDD TKTLIKTIVT RINDISHTQS VSARQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQILTSLP SQNVLQIAHD LENLRDLLHL LAFSKSCSLP QTRGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDLSPEC |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Recombinant Rat Leptin was lyophilized from a.2 μm filteredNaHCO3 solution. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |