Rat Leptin Recombinant

Rat Leptin Recombinant

$70.00$450.00


accession P50596


Source Optimized DNA sequence encodingRatLeptin mature chain was expressed in Escherichia Coli.
Molecular weight RatLeptin is generated by the proteolytic removal of the signal peptideand propeptide, the molecule has a calculated molecular mass of approximately16 kDa. Recombinant Leptin is a disulfide-linked homodimeric protein consisting of amino acid residue subunits, and migrates as an approximately16 kDa protein under non-reducingand reducing conditions in SDS-PAGE.
Purity >98%, as determined by SDS-PAGE and HPLC
Biological Activity Activitywas determined by the dose-dependent proliferation ofBAF/3 cells.
Protein Sequence MCWRPLCRFL WLWSYLSYVQ AVPIHKVQDD TKTLIKTIVT RINDISHTQS VSARQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQILTSLP SQNVLQIAHD LENLRDLLHL LAFSKSCSLP QTRGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDLSPEC
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant Rat Leptin was lyophilized from a.2 μm filteredNaHCO3 solution.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.