Rat Leptin Recombinant

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$70.00$450.00

SKU: RKP50596 Tags: , , , ,

Description

Accession
P50596
Source
Optimized DNA sequence encodingRatLeptin mature chain was expressed in Escherichia Coli.
Molecular weight
Rat Leptin is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately1 16 kDa. Recombinant Leptin is a monomer protein consisting of 147 amino acid residue subunits, and migrates as an approximately 16 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>98%, as determined by SDS-PAGE and HPLC
Biological Activity
Activitywas determined by the dose-dependent proliferation ofBAF/3 cells.
Protein Sequence
MCWRPLCRFL WLWSYLSYVQ AVPIHKVQDD TKTLIKTIVT RINDISHTQS VSARQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQILTSLP SQNVLQIAHD LENLRDLLHL LAFSKSCSLP QTRGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDLSPEC
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant Rat Leptin was lyophilized from a.2 μm filteredNaHCO3 solution.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
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