Rat Interleukin-6 Recombinant
$70.00 – $4,700.00
accession | P20607 |
Source | Optimized DNA sequence encoding rat Interleukin-6 mature chain was expressed in Escherichia Coli. |
Molecular weight | Native rat Interleukin-6 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 22kDa. Recombinant rat Interleukin-6 is a monomer protein consisting of 188 amino acid residue subunits, and migrates as an approximately 22kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. |
Purity | >95%, as determined by SDS-PAGE and HPLC |
Biological Activity | The ED(50) was determined by the dose-dependent stimulation of the proliferation of monkeyMBr-5 cells was found to be in the range of.0-40.0 ng/ml. |
Protein Sequence | MKFLSARDFQ PVAFLGLMLL TATAFPTSQV RRGDFTEDTT HNRPVYTTSQ VGGLITYVLR EILEMRKELC NGNSDCMNSD DALSENNLKL PEIQRNDGCF QTGYNQEICL LKICSGLLEF RFYLEFVKNN LQDNKKDKAR VIQSNTETLV HIFKQEIKDS YKIVLPTPTS NALLMEKLES QKEWLRTKTI QLILKALEEF LKVTMRSTRQ T |
Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
Presentation | Recombinant rat Interleukin-6 was lyophilized from a.2 μm filtered PBS solution. |
Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
Biological Process | Acute-phase |
Molecular function | Cytokine |
Molecular function | Growth-factor |
Methods
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2.5. Primary splenocyte and bone marrow-derived dendritic cell (BMDC) culture
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