Rat Interleukin-6 Recombinant

HomeHematopoietins, IL-6 gp130 family, Recombinant Rat Cytokines

Rat Interleukin-6 Recombinant

$70.00 – $4,700.00

SKU: RKP20607 Categories: Hematopoietins, IL-6 gp130 family, Recombinant Rat Cytokines Tags: il-6, il6, interleukin-6, P20607

Description

Methods (2)


accession	P20607

Source	Optimized DNA sequence encoding rat Interleukin-6 mature chain was expressed in Escherichia Coli.
Molecular weight	Native rat Interleukin-6 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 22kDa. Recombinant rat Interleukin-6 is a monomer protein consisting of 188 amino acid residue subunits, and migrates as an approximately 22kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Purity	>95%, as determined by SDS-PAGE and HPLC
Biological Activity	The ED(50) was determined by the dose-dependent stimulation of the proliferation of monkeyMBr-5 cells was found to be in the range of.0-40.0 ng/ml.
Protein Sequence	MKFLSARDFQ PVAFLGLMLL TATAFPTSQV RRGDFTEDTT HNRPVYTTSQ VGGLITYVLR EILEMRKELC NGNSDCMNSD DALSENNLKL PEIQRNDGCF QTGYNQEICL LKICSGLLEF RFYLEFVKNN LQDNKKDKAR VIQSNTETLV HIFKQEIKDS YKIVLPTPTS NALLMEKLES QKEWLRTKTI QLILKALEEF LKVTMRSTRQ T
Endotoxin	Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation	Recombinant rat Interleukin-6 was lyophilized from a.2 μm filtered PBS solution.
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage	The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Biological Process	Acute-phase
Molecular function	Cytokine
Molecular function	Growth-factor

Methods


Chemicals and Kits The biochemical tests conducted with the specific enzymatic colorimetric assay kits were all provided by, either located at Switzerland or . The reagents IFCC and P-5-P were used for assay of GOT and GPT. Octacalcium phosphate (OCP) and UV were used for the determination of serum calcium and phosphate ions. For other determinations we used BCG for serum albumin, CHOD for cholesterol, lipase-glycerol oxidase for triglycerides; KINETIC for serum BUN, picric acid for creatinine (reaction), colorimetric oxidase for uric acid, and Sirius Red for collagen staining. Doxorubicin (DR) was a product of Pfizer . The kits for other determinations included SO and TBAS from Cayman (Michigan, ), the rat IL-6 EIA Kit from , and the rat TNF-α Kit from the& . The sources of the antibodies used in this experiment were: PDGF Receptor β (1∶1000), phosphor-PDGF Receptor β (1∶1000) and β-actin from Cell Signaling ; phosphor-PI3K (1∶500) from Santa… The biochemical tests conducted with the specific enzymatic colorimetric assay kits were all provided by, either located at Switzerland or . The reagents IFCC and P-5-P were used for assay of GOT and GPT. Octacalcium phosphate (OCP) and UV were used for the determination of serum calcium and phosphate ions. For other determinations we used BCG for serum albumin, CHOD for cholesterol, lipase-glycerol oxidase for triglycerides; KINETIC for serum BUN, picric acid for creatinine (reaction), colorimetric oxidase for uric acid, and Sirius Red for collagen staining. Doxorubicin (DR) was a product of Pfizer . The kits for other determinations included SO and TBAS from Cayman (Michigan, ), the rat IL-6 EIA Kit from , and the rat TNF-α Kit from the& . The sources of the antibodies used in this experiment were: PDGF Receptor β (1∶1000), phosphor-PDGF Receptor β (1∶1000) and β-actin from Cell Signaling ; phosphor-PI3K (1∶500) from Santa Cruz ; α-Smooth Muscle Actin (1∶1000) from ; CD34 (1∶400) from . Chemiluminescent HRP Substrate was the product of . Sodium dodecyl sulfate (SDS) and polyacrylamide gel (PAGE) were products of . Read more
2.5. Primary splenocyte and bone marrow-derived dendritic cell (BMDC) culture Untreated 6-week-old SD rats were sacrificed by cervical dislocation after ether exposure. Cells in the spleen were harvested and prepared as a single cell suspension in complete medium in a 24-well plate (1×10⁷ cells/well). In the control group, splenocytes were incubated alone or with recombinant human TGF-ß (2 ng/ml, , , ) and recombinant rat IL-6 (20 ng/ml, , , ) at 37°C for 72 h. For the experimental groups, in addition to TGF-ß and IL-6, cells were treated with culture supernatant of F. prausnitzii, sodium butyrate (0.05834 µmol/well), denatured F. prausnitzii and B. longum bacteria (1× 10⁷ CFU/well). Each group treatment was repeated four times. After 72 h, the supernatant of cultured splenocytes was collected and stored at −20°C for further analysis