Rat Interleukin-5 Recombinant

Rat Interleukin-5 Recombinant

$70.00$4,700.00


accession Q08125


Source Optimized DNA sequence encoding Rat Interleukin-5 mature chain was expressed in Escherichia Coli.
Molecular weight Native Rat Interleukin-5, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 13 kDa. Recombinant IL-5 is a disulfide-linked homodimeric protein consisting of two 113 amino acid residue subunits,and migrates as an approximately 26 kDa protein under non-reducing conditions and as a 13 kDa protein under reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity The ED(50) was determined by the dose-dependent proliferation of TF-1 cells was ≤.2 ng/ml, corresponding to a specific activity of ≥ x units/mg.
Protein Sequence MRMLLCLNVL TLSCVWAIAM EIPMSTVVKE TLIQLSTHRA LLTSNETMRL PVPTHKNHQL CIGEIFQGLD ILKNQTVRGG TVEILFQNLS LIKKYIDGQK EKCGEERRKT RHFLDYLQEF LGVMSTEWAM EV
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant rat Interleukin-5 was lyophilized from a.2 μm filtered PBS solution.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only. It may not be used for therapeutics or diagnostic purposes.


Molecular function Cytokine
Molecular function Growth-factor

Methods

γIMCs are the source of IFN-γ in severe invasive GAS infections.

IL-5
  • (f) Sorted IMCs were cultured with control medium (Med), G-CSF (50 ng ml−1), M-CSF (10 ng ml−1), GM-CSF (10 ng ml−1), or IL-5 (10 ng ml−1) for 2–4 days, and their absolute numbers were counted on the indicated days.