/, IL-2 family, Recombinant Rat Cytokines/Rat Interleukin-15 Recombinant

Rat Interleukin-15 Recombinant

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$70.00$3,500.00

SKU: RKP97604 Tags: , , ,

Description

Accession
P97604
Source
Optimized DNA sequence encoding rat Interleukin-15 mature chain was expressed in Escherichia Coli.
Molecular weight
Native rat Interleukin-15 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 14 kDa. Recombinant rat-15 is a monomer protein consisting of 115 amino acid residue subunits, and migrates as an approximately 14kDa protein under reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the dose-dependent proliferation of murine CTLL-2 was found to be in the range of.0 ng/ml.

Protein Sequence
MKILKPYMRN TSILYYLCFL LNSHFLTEAG IHVFILGCVS VGLPKTEANW IDVRYDLEKI ESLIQFIHID TTLYTDSDFH PSCKVTAMNC FLLELQVILH EYSNMTLNET VRNVLYLANS TLSSNKNVIE SGCKECEELE ERNFTEFLQS FIHIVQMFIN TS
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant rat Interleukin-15 was lyophilized from a 0.2 μm filtered PBS solution.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function

Methods

Activation of NK cells after exposure to B16 is supported by IL-15.

  • B. IL-15 supports NK cell activation.
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