Rat Insulin Like Growth Factor-I Recombinant
$70.00 – $900.00
| accession | P08025 |
| Source | Optimized DNA sequence encoding Rat Insulin Growth Factor -1mature chain was expressed in Escherichia Coli. |
| Molecular weight | Native Rat Insulin Growth Factor-1 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 8kDa. Recombinant rat IGF-1 is a monomeric protein consisting of 70 amino acid residue subunits. IGF-1 migrates as an approximately 8kDa protein under non-reducing and reducing conditions in SDS-PAGE. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | The ED50 was determined by a cell proliferation assay using FDC-P1 cells is ≤.0 ng/ml, corresponding to a specific activity of ≥1 x units/mg. |
| Protein Sequence | MGKISSLPTQ LFKCCFCDFL KVKMHTMSSS HLFYLALCLL TFTSSATAGP ETLCGAELVD ALQFVCGPRG FYFNKPTGYG SSIRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPTKSA RS VRAQRHTDMP KTQKEVHLKN ASRGSAGNKN YRM |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Recombinant Rat IGF1was lyophilized from a.2 μm filtered PBS solution, pH.4. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Molecular function | Growth-factor |
Methods
Impaired IGF1 signaling in fibroblasts that have phenotypic features of diabetes, inflammation or hypercortisolemia.
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