Rat Insulin Like Growth Factor-I Recombinant

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Rat Insulin Like Growth Factor-I Recombinant

$70.00$900.00

SKU: RKP08025 Categories: , Tags: , , , ,

accession P08025


Source Optimized DNA sequence encoding Rat Insulin Growth Factor -1mature chain was expressed in Escherichia Coli.
Molecular weight Native Rat Insulin Growth Factor-1 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 8kDa. Recombinant rat IGF-1 is a monomeric protein consisting of 70 amino acid residue subunits. IGF-1 migrates as an approximately 8kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity The ED50 was determined by a cell proliferation assay using FDC-P1 cells is ≤.0 ng/ml, corresponding to a specific activity of ≥1 x units/mg.
Protein Sequence MGKISSLPTQ LFKCCFCDFL KVKMHTMSSS HLFYLALCLL TFTSSATAGP ETLCGAELVD ALQFVCGPRG FYFNKPTGYG SSIRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPTKSA RS VRAQRHTDMP KTQKEVHLKN ASRGSAGNKN YRM
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant Rat IGF1was lyophilized from a.2 μm filtered PBS solution, pH.4.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Molecular function Growth-factor

Methods

Impaired IGF1 signaling in fibroblasts that have phenotypic features of diabetes, inflammation or hypercortisolemia.

IGF1
  • Fibroblasts with phenotypic characteristics of type 2 diabetes (Diab) and those of control fibroblasts treated chronically with either vehicle (Cont), TNFα (4 ng/ml every day for 4 days) or dexamethasone (Dexa; 20 ng/ml every other day for 8 days) were serum starved for 24 hours prior to treatment for 15 minutes with IGF1 (50 ng/ml).