Rat GRO-gamma alpha (CXCL3) Recombinant

///Rat GRO-gamma alpha (CXCL3) Recombinant

Rat GRO-gamma alpha (CXCL3) Recombinant

$70.00$2,700.00

SKU: RKQ10746 Category: Tags: , , , ,

accession Q10746


Source Optimized DNA sequence encoding rat GRO-gamma/CXCL3 mature chain was expressed in Escherichia Coli
Molecular weight Native rat GROgamma/CXCL3 is generated by the proteolytic removal of the signal peptide and propeptide. The molecule has a calculated molecular mass of approximately 7kDa. Recombinant GRO-gamma is a disulfide-linked homodimeric protein consisting of 70amino acid residue subunits, and migrates as an approximately 7kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity Determined by its ability to chemoattract transfected CXCR2 cells using a concentration range of-100 ng/ml.

Protein Sequence MGEIRVDNFS LEDFFSGDID SYNYSSDPPF TLSDAAPCPS ANLDINRYAV VVIYVLVTLL SLVGNSLVML VILYNRSTCS VTDVYLLNLA IADLFFALTL PVWAASKVNG WIFGSFLCKV FSFLQEITFY SSVLLLACIS MDRYLAIVHA TSTLIQKRHL VKFVCITMWF LSLVLSLPIF ILRTTVKANP STVVCYENIG NNTSKWRVVL RILPQTYGFL LPLLIMLFCY GFTLRTLFKA HMGQKHRAMR VIFAVVLVFL LCWLPYNIVL FTDTLMRTKL IKETCERQNE INKALEATEI LGFLHSCLNP IIYAFIGQKF RHGLLKIMAN YGLVSKEFLA KEGRPSFVGS SSANTSTTL
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant mouse GRO-gamma/CXCL3 was lyophilized from a.2μm filtered concentrated (1mg/ml) solution inmM NaCl,mM PB, pH7.0.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Biological Process Chemotaxis
Biological Process Inflammatory-response
Molecular function Cytokine