Rat GRO-beta (CXCL2) Recombinant

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Rat GRO-beta (CXCL2) Recombinant

$70.00$2,700.00


accession P30348


Source Optimized DNA sequence encoding rat GRO-beta CXCL2 mature chain was expressed in Escherichia Coli.
Molecular weight Native rat GRO-beta is generated by the proteolytic removal of the signal peptide and propeptide. The molecule has a calculated molecular mass of approximately 8.5kDa. Recombinant GRO-beta is a disulfide-linked homodimeric protein consisting of 68 amino acid residue subunits, and migrates as an approximately 8.5kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity Determined by its ability to chemoattract rat neutrophils cells using a concentration range of-100 ng/ml.

Protein Sequence MAPPTRQLLN AVLVLLLLLA TNHQGTGVVV ASELRCQCLT TLPRVDFKNI QSLTVTPPGP HCAQTEVIAT LKDGHEVCLN PEAPLVQRIV QKILNKGKAN
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant rat GRO-beta/CXCL2 was lyophilized from a.2μm filtered concentrated (1mg/ml) solution inPBS, pH.4.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Biological Process Chemotaxis
Biological Process Inflammatory-response
Molecular function Cytokine

Methods

Recombinant chemokines increase axon morphogenesis.

CXCL2
  • Hippocampal neurons (2 DIV) control or treated with CXCL2, CCL5, CCL20, and CCL7 (1000 ng/ml) and immunostained for βIII-tubulin to reveal the axon morphology.