/, CXC chemokines/Rat GRO-beta (CXCL2) Recombinant

Rat GRO-beta (CXCL2) Recombinant

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$70.00$2,700.00

SKU: RKP30348 Tags: , , , , , ,

Description

Accession
P30348
Source
Optimized DNA sequence encoding rat GRO-beta CXCL2 mature chain was expressed in Escherichia Coli.
Molecular weight
Native rat GRO-beta is generated by the proteolytic removal of the signal peptide and propeptide. The molecule has a calculated molecular mass of approximately 8.5kDa. Recombinant GRO-beta is a monomer protein consisting of 73 amino acid residue subunits, and migrates as an approximately 8.5kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
Determined by its ability to chemoattract rat neutrophils cells using a concentration range of-100 ng/ml.

Protein Sequence
MAPPTRQLLN AVLVLLLLLA TNHQGTGVVV ASELRCQCLT TLPRVDFKNI QSLTVTPPGP HCAQTEVIAT LKDGHEVCLN PEAPLVQRIV QKILNKGKAN
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant rat GRO-beta/CXCL2 was lyophilized from a.2μm filtered concentrated (1mg/ml) solution inPBS, pH.4.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Biological Process
Molecular function

Methods

Recombinant chemokines increase axon morphogenesis.

  • Hippocampal neurons (2 DIV) control or treated with CXCL2, CCL5, CCL20, and CCL7 (1000 ng/ml) and immunostained for βIII-tubulin to reveal the axon morphology.
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