Rat basic Fibroblast Growth Factor Recombinant
Categories: FGF familyRecombinant Rat Cytokines$70.00 – $1,100.00
Description
Accession
P13109
Source
Optimized DNA sequence encoding Rat basic Fibroblast Growth Factor mature chain was expressed in Escherichia Coli.
Molecular weight
Native Rat FGF basic is generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calulated mass of kDa. Recombinant Rat bFGF is a monomer protein consisting of 154 amino acid residue subunits, and migrates as an approximately 16 kDa protein under reducing conditions in SDS-PAGE.
Purity
>96%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) determined by dose-dependent stimulation of thymidine uptake byT3 cells expressing FGF receptors, was ofund to be <1 ng/ml.
Protein Sequence
MAAGSITSLP ALPEDGGGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHVK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTEEC FFFERLESNN YNTYRSRKYS SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Rat Basic FGF was lyophilized from a.2 μm filtered PBS solution pH7.5 .
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Biological Process
Molecular function
Molecular function
Molecular function
