Name: Rabbit Anti Rat IL-1b polyclonal, antigen affinity
SKU: RKAP100
Availability: InStock


accession	Q63264

Applications	ELISA:This antibody can be used at 2 μg/mL jointly with biotinylated Rat IL-1b antibody to detect Rat IL-1b. The detection limit for recombinant Rat IL-1b is approximately 0.2 ng/well. Western Blot:This antibody can be used at 0.2 μg/mL with the appropriate secondary reagents to detect Rat IL-1b. The detection limit for recombinant Rat IL-1b is approximately 0.2 ng/lane.
Source	This antibody was produced in rabbits immunized with recombinant Rat IL-1b.
Species reactivity	Rat
Purification	The specific antibody was purified from Rabbit sera by using immobilized recombinant Rat IL-1b affinity chromatography.
Presentation	Lyophilized from PBS, pH 7.2.
Storage	The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C. Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for 12 months without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Biological Process	Inflammatory-response
Molecular function	Cytokine
Molecular function	Mitogen
Molecular function	Pyrogen


Sandwich ELISA 96-well maxisorp plates were coated with the capture antibody overnight, then blocked with 5% FCS in PBS for 1 hour, then washed. Then, the protein standard was added, and plates were incubated for 2 hrs at 37°C. Plates were washed and incubated with a biotinylated detection antibody for 1 hour at 37°C. After washing, plates were incubated with HRP-conjugated streptavidin for 30 min at room temperature and washed again. Plates were developed using the tetramethylbenzidine peroxidase substrate system and absorbance was measured at 450-615 nm using an automated plate reader.
Western Blot The Recombinant immunogen was migrated by 12% SDS-PAGE and transferred to a 0.2 um PVDF membrane. After blocking the membrane in blocking buffer (5% milk powder in 20 mM Tris-HCl pH 7.5, 500 mM NaCl, 0.1% (v/v) Tween 20 , the membrane was incubated with primary antibody ( at 4°C overnight. Peroxidase-linked secondary anti-rabbit were used to detect the bound primary antibodies. Enhanced chemiluminescence (ECL) reagents were used to visualize

Rabbit Anti Rat IL-1b polyclonal, antigen affinity

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