Rabbit Anti Mouse B7-1 polyclonal, antigen affinity

///Rabbit Anti Mouse B7-1 polyclonal, antigen affinity

Rabbit Anti Mouse B7-1 polyclonal, antigen affinity

$150.00$190.00

SKU: RKAP008 Category: Tags: , , ,

accession Q00609


Applications

ELISA:This antibody can be used as capture antibody at 2 μg/mL jointly with biotinylated Mouse B7-1 antibody to detect Mouse B7-1. The detection limit for recombinant Mouse B7-1 is approximately 0.2 ng/well.

Western Blot:This antibody can be used at 0.2 μg/mL with the appropriate secondary reagents to detect Mouse B7-1. The detection limit for recombinant Mouse B7-1 is approximately 0.2 ng/lane.

Source This antibody was produced in rabbits immunized with recombinant Mouse B7-1.
Species reactivity Mouse
Purification The specific antibody was purified from Rabbit sera by using immobilized recombinant Mouse B7-1 affinity chromatography.
Presentation Lyophilized from PBS, pH 7.2.
Storage The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C. Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for 12 months without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Molecular function Receptor

Methods

Western Blot

  • The Recombinant immunogen was migrated by 12% SDS-PAGE and transferred to a 0.2 um PVDF membrane.
  • After blocking the membrane in blocking buffer (5% milk powder in 20 mM Tris-HCl pH 7.5, 500 mM NaCl, 0.1% (v/v) Tween 20 , the membrane was incubated with primary antibody ( at 4°C overnight.
  • Peroxidase-linked secondary anti-rabbit were used to detect the bound primary antibodies.
  • Enhanced chemiluminescence (ECL) reagents were used to visualize