Rabbit Anti Human NGFb polyclonal, antigen affinity

///Rabbit Anti Human NGFb polyclonal, antigen affinity

Rabbit Anti Human NGFb polyclonal, antigen affinity

$150.00$190.00

SKU: RKAP208 Category: Tags: , , ,

accession P01138


Applications

ELISA:This antibody can be used at 2 μg/mL jointly with biotinylated Human NGFb antibody to detect Human NGFb. The detection limit for recombinant Human NGFb is approximately 0.2 ng/well.

Western Blot:This antibody can be used at 0.2 μg/mL with the appropriate secondary reagents to detect Human NGFb. The detection limit for recombinant Human NGFb is approximately 0.2 ng/lane.

Source This antibody was produced in Rabbit immunized with recombinant Human NGFb.
Species reactivity Human
Purification The specific antibody was purified from Rabbit sera by using immobilized recombinant Human NGFb affinity chromatography.
Presentation Lyophilized from PBS, pH 7.2.
Storage The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C. Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for 12 months without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P04629 NTRK1_HUMAN
Interactor P35739 NTRK1_RAT
Interactor Q99523
Interactor P07174
Interactor P08138 TNR16_HUMAN
Molecular function Growth-factor
Molecular function Metalloenzyme-inhibitor
Molecular function Protease-inhibitor

Methods

Sandwich ELISA

  • 96-well maxisorp plates were coated with the capture antibody overnight, then blocked with 5% FCS in PBS for 1 hour, then washed.
  • Then, the protein standard was added, and plates were incubated for 2 hrs at 37°C.
  • Plates were washed and incubated with a biotinylated detection antibody for 1 hour at 37°C.
  • After washing, plates were incubated with HRP-conjugated streptavidin for 30 min at room temperature and washed again.
  • Plates were developed using the tetramethylbenzidine peroxidase substrate system and absorbance was measured at 450-615 nm using an automated plate reader.

Western Blot

  • The Recombinant immunogen was migrated by 12% SDS-PAGE and transferred to a 0.2 um PVDF membrane.
  • After blocking the membrane in blocking buffer (5% milk powder in 20 mM Tris-HCl pH 7.5, 500 mM NaCl, 0.1% (v/v) Tween 20 , the membrane was incubated with primary antibody ( at 4°C overnight.
  • Peroxidase-linked secondary anti-rabbit were used to detect the bound primary antibodies.
  • Enhanced chemiluminescence (ECL) reagents were used to visualize