Rabbit Anti Human IL-15 polyclonal, antigen affinity

///Rabbit Anti Human IL-15 polyclonal, antigen affinity

Rabbit Anti Human IL-15 polyclonal, antigen affinity

$150.00$190.00

SKU: RKAP134 Category: Tags: , , ,

accession P40933


Applications

ELISA:This antibody can be used at 2 μg/mL jointly with biotinylated Human IL-15 antibody to detect Human IL-15. The detection limit for recombinant Human IL-15 is approximately 0.2 ng/well.

Western Blot:This antibody can be used at 0.2 μg/mL with the appropriate secondary reagents to detect Human IL-15. The detection limit for recombinant Human IL-15 is approximately 0.2 ng/lane.

Source This antibody was produced in rabbits immunized with recombinant Human IL-15.
Species reactivity Human
Purification The specific antibody was purified from Rabbit sera by using immobilized recombinant Human IL-15 affinity chromatography.
Presentation Lyophilized from PBS, pH 7.2.
Storage The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C. Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for 12 months without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P14784 IL2RB_HUMAN
Interactor P31785 IL2RG_HUMAN
Interactor P41273 TNFL9_HUMAN
Molecular function Cytokine

Methods

Sandwich ELISA

  • 96-well maxisorp plates were coated with the capture antibody overnight, then blocked with 5% FCS in PBS for 1 hour, then washed.
  • Then, the protein standard was added, and plates were incubated for 2 hrs at 37°C.
  • Plates were washed and incubated with a biotinylated detection antibody for 1 hour at 37°C.
  • After washing, plates were incubated with HRP-conjugated streptavidin for 30 min at room temperature and washed again.
  • Plates were developed using the tetramethylbenzidine peroxidase substrate system and absorbance was measured at 450-615 nm using an automated plate reader.

Western Blot

  • The Recombinant immunogen was migrated by 12% SDS-PAGE and transferred to a 0.2 um PVDF membrane.
  • After blocking the membrane in blocking buffer (5% milk powder in 20 mM Tris-HCl pH 7.5, 500 mM NaCl, 0.1% (v/v) Tween 20 , the membrane was incubated with primary antibody ( at 4°C overnight.
  • Peroxidase-linked secondary anti-rabbit were used to detect the bound primary antibodies.
  • Enhanced chemiluminescence (ECL) reagents were used to visualize