Rabbit Anti Human IFNG polyclonal, antigen affinity

///Rabbit Anti Human IFNG polyclonal, antigen affinity

Rabbit Anti Human IFNG polyclonal, antigen affinity

$150.00$190.00


accession P01579


Applications

ELISA:This antibody can be used at 2 μg/mL jointly with biotinylated Human IFNG antibody to detect Human IFNG. The detection limit for recombinant Human IFNG is approximately 0.2 ng/well.

Western Blot:This antibody can be used at 0.2 μg/mL with the appropriate secondary reagents to detect Human IFNG. The detection limit for recombinant Human IFNG is approximately 0.2 ng/lane.

Source This antibody was produced in rabbits immunized with recombinant Human IFNG.
Species reactivity Human
Purification The specific antibody was purified from Rabbit sera by using immobilized recombinant Human IFNG affinity chromatography.
Presentation Lyophilized from PBS, pH 7.2.
Storage The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C. Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for 12 months without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P15260 INGR1_HUMAN
Interactor Q66793
Interactor P70380 IL18_MOUSE
Biological Process Antiviral-defense
Biological Process Growth-regulation
Molecular function Cytokine

Methods

T. crassiceps-inoculation induces elevation of IL-4 and hepatic expression of Ym-1.

IFN-γ capture antibodies
  • Six weeks post-inoculation, IL-4 and IFN-γ serum levels were determined from Tc-inoculated and non-inoculated mice by ELISA.

Sandwich ELISA

  • 96-well maxisorp plates were coated with the capture antibody overnight, then blocked with 5% FCS in PBS for 1 hour, then washed.
  • Then, the protein standard was added, and plates were incubated for 2 hrs at 37°C.
  • Plates were washed and incubated with a biotinylated detection antibody for 1 hour at 37°C.
  • After washing, plates were incubated with HRP-conjugated streptavidin for 30 min at room temperature and washed again.
  • Plates were developed using the tetramethylbenzidine peroxidase substrate system and absorbance was measured at 450-615 nm using an automated plate reader.

Western Blot

  • The Recombinant immunogen was migrated by 12% SDS-PAGE and transferred to a 0.2 um PVDF membrane.
  • After blocking the membrane in blocking buffer (5% milk powder in 20 mM Tris-HCl pH 7.5, 500 mM NaCl, 0.1% (v/v) Tween 20 , the membrane was incubated with primary antibody ( at 4°C overnight.
  • Peroxidase-linked secondary anti-rabbit were used to detect the bound primary antibodies.
  • Enhanced chemiluminescence (ECL) reagents were used to visualize