ELISA: Use at a concentration range of 1-2ug/ml.
Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 µg/ml.IHC:A suitable range of concentrations of this antibody for IHC is 2-10 µg/ml. Immunohistochemical analysis of CD31-positive microvessels in FFPE Human malignant melanoma cell line, A375. Cat#RPA16284 was used as primary antibody, dilution 1:200, DAB chromogen substrate. Immunohistochemical analysis of mouse CD31 expression in FFPE vascular endothelial melanoma cell. Cat#RPA16284 was used as primary antibody, dilution 1:200, DAB chromogen substrate. .
|Source||PolyclonalCD31 antibody was produced from sera of rabbits immunized with synthetic protein containing CD31 antigenic determinants.|
|Purification||Serum IgG fraction was purified by Protein G affinity chromatography.|
|Presentation||Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.|
|Reconstitution||A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers|
|Storage||The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C. Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles.|
|Usage||This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.|
Rabbit anti CD31 (PECAM1) Polyclonal
$190.00 – $490.00