Mouse TNF-alpha Recombinant
$70.00 – $2,700.00
|Source||Optimized DNA sequence encoding Mouse Tumor Necrosis Factor-alpha extracellular domain was expressed in Escherichia Coli.|
|Molecular weight||Native Mouse Tumor Necrosis Factor-alpha is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated mass of approximately 17kDa. Recombinant Mouse TNF is a monomer protein,consisting of 157 amino acids and migrates as an approximately17 kDa protein under reducing conditions.|
|Purity||>97%, as determined by SDS-PAGE and HPLC|
|Biological Activity||The ED(50) was determinedcytolysis of murine L929 cells in the presence of Actinomycin D is ≤.05 ng/ml, corresponding to a specific activity of ≥3 x units/mg.|
|Protein Sequence||MSTESMIRDV ELAEEALPQK MGGFQNSRRC LCLSLFSFLL VAGATTLFCL LNFGVIGPQR DEKFPNGLPL ISSMAQTLTL RSSSQNSSDK PVAHVVANHQ VEEQLEWLSQ RANALLANGM DLKDNQLVVP ADGLYLVYSQ VLFKGQGCPD YVLLTHTVSR FAISYQEKVN LLSAVKSPCP KDTPEGAELK PWYEPIYLGG VFQLEKGDQL SAEVNLPKYL DFAESGQVYF GVIAL|
|Endotoxin||Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).|
|Presentation||Recombinant mouse TNF alpha was lyophilized from.2 μm filtered PBS solution, pH7.0.|
|Reconstitution||A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.|
|Storage||The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.|
|Usage||This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.|
Anti-inflammatory effects of diazoxide pre-treatment in microglial cell cultures stimulated with LPS and IFNγ.
Flow cytometric analysis of endothelial VCAM-1 and E-selectin
Enhanced circulating and intrathymic contents of TNF-α parallel the thymic atrophy in T. cruzi acutely-infected mice.
Transendothelial Migration and Invadopodia (in Vitro Filipodia) Formation Assay of the T Lymphoma Cells