Mouse SIRP alpha Recombinant
Category: Surface & Soluble Receptors$160.00 – $1,950.00
Description
Accession
P97797
Source
Optimized DNA sequence encodingMouse Signal regulatory protein alpha - SIRP alpha (SHPS1)(THR32 - Asn 373) extracellular domain including a C-terminal His tag was expressed in HEK293 cells.
Molecular weight
RecombinantMouseSIRP alpha (SHPS1) is a protein consisting of 350 amino acid residue subunits,due to glycosylation migrates as an approximately as 50kDa band protein on reduced SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The activity was determined by the ability of immobilized mouse SIRPA to bind recombinant human CD47. The ED50 was calculated to be < 70ng/ml
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
RecombinantMouseSIRP alpha (SHPS1) is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
RecombinantMouseSIRP alpha (SIRPA) can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
