Mouse SDF-1 alpha Recombinant
$70.00 – $2,700.00
accession | P40224 |
Source | Optimized DNA sequence encoding Mouse SDF-1 alpha mature chain was expressed in Escherichia Coli. |
Molecular weight | Native Mouse Stromal Cell-Derived Factor-1 alpha, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately 8 kDa. Recombinant SDF-1 alpha is a monomer protein consisting of 69 amino acid residue subunits, migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE. |
Purity | >95%, as determined by SDS-PAGE and HPLC |
Biological Activity | Determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentration range of-40 ng/ml. |
Protein Sequence | MDAKVVAVLA LVLAALCISD GKPVSLSYRC PCRFFESHIA RANVKHLKIL NTPNCALQIV ARLKNNNRQV CIDPKLKWIQ EYLEKALNK |
Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
Presentation | Recombinant SDF-1 alpha was lyophilized from a.2 μm filtered solution in.5% glycine,.5% sucrose,.01% Tween80, mM Glutamic acid, pH.5. |
Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
Interactor | Q8JLJ0 |
Interactor | Q8QMN0 |
Interactor | Q9JFT2 |
Biological Process | Chemotaxis |
Molecular function | Cytokine |
Molecular function | Growth-factor |
Methods
ELISA
|
Cell Migration
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