Description
Accession
Q99P87
Source
Optimized DNA sequence encoding mouse Resistin mature chain was expressed in Escherichia Coli.
Molecular weight
Native mouse Resistin, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 20 kDa. Recombinant Resistin is a monomer consisting of 95 amino acid residue subunits,and migrates as an approximately 20 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
Determined by its ability to stimulate lipolysis in cultured human adipocytes.
Protein Sequence
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant mouse Resistin was lyophilized from a 0.2 μm filtered PBS solution.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
