Mouse RANTES-CCL5 Recombinant

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Mouse RANTES-CCL5 Recombinant

$70.00$2,700.00


accession P30882


Source Optimized DNA sequence encoding Mouse RANTES mature chain was expressed in Escherichia Coli.
Molecular weight Native mouse RANTES is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 8 kDa. Recombinant RANTES is a monomer protein consisting of 68 amino acid residue subunits and migrates as an approximately 8 kDa protein under non-reducingand reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity The ED(50) was determined by the ability to chemoattract human lymphocyte population using a concentration range of 0.0-10.0 ng/ml.

Protein Sequence MKISAAALTI ILTAAALCTP APASPYGSDT TPCCFAYLSL ALPRAHVKEY FYTSSKCSNL AVVFVTRRNR QVCANPEKKW VQEYINYLEM S
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant mouse RANTES/CCL5 was lyophilized from a.2 μm filtered PBS solution.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Biological Process Chemotaxis
Biological Process Inflammatory-response
Molecular function Cytokine

Methods

CCL5-induced tumor-promoting properties in CRC cells.

recombinant CCL5
  • Proliferation of the CT26 and HT29 cells was assessed in response to a 5-day treatment with base medium alone (BSA, open bars), with serum-enriched medium (FBS, hatched bars) or with the indicated concentrations of recombinant CCL5 (filled bars), in the presence or in the absence of TAK-779 or anti-CCL5 antibodies (at the indicated concentrations).

Ccr1 ligands are induced after Candida infection and are chemotactic for kidney neutrophils ex vivo.

CCL5
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Binding and in vitro activity of murine 18V4F hybridoma antibody.

CCL5
  • Chemotaxis inhibition by 18V4F hybridoma antibody of CCR5-transfected Ba/F3 cells to 5 ng/mL of CCL3, CCL4, and CCL5.

Binding and in vitro activity of murine 18V4F hybridoma antibody.

CCL5
  • Chemotaxis inhibition by 18V4F hybridoma antibody of CCR5-transfected Ba/F3 cells to 5 ng/mL of CCL3, CCL4, and CCL5.

Validation of the array expression pattern by quantitative RT-PCR.

CCL5/RANTES
  • The mRNA levels of CCL2/MCP-1 , CCL5/RANTES , ICAM-1 , VCAM-1 , E-selectin , and P-selectin were determined by quantitative real-time PCR, and values were normalized to 18S rRNA as reference gene.

Induction of ORMDL3 and CD48 expression

  • BM-derived eosinophils (1 × 106/well) were suspended in culture medium alone or medium containing IL-5, IL-3 (both from)ANTES-1 (& all at 100 ng/ml) or eotaxin-1 (100 nM) for 2 h at 37°C and 5% CO2 after which cells were analyzed for OML3 mNA expression byT-PC and qPC.
  • To evaluate CD48 mRNA expression, eosinophils were treated with IL-3 as described above.
  • For protein expression, eosinophils were treated with IL-3 for 12 h and then examined for ORMDL3 and CD48 by Western blot analysis followed by densitometry using ImageJ (for ORMDL3).