Description
Accession
P06879
Source
Optimized DNA sequence encoding Mouse Prolactin mature chain was expressed in Escherichia Coli.
Molecular weight
Native mouse Prolactin is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 23kDa. Recombinant Prolcatin is a monomeric protein consisting of 198 amino acid residue subunits, and migrates as an approximately 23 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>98%, as determined by SDS-PAGE and HPLC
Biological Activity
The activity was determined by the dose-dependent proliferation of Nb2 cells.
Protein Sequence
MNSQGSAQKA GTLLLLLISN LLFCQNVQPL PICSAGDCQT SLRELFDRVV ILSHYIHTLY TDMFIEFDKQ YVQDREFMVK VINDCPTSSL ATPEDKEQAL KVPPEVLLNL ILSLVQSSSD PLFQLITGVG GIQEAPEYIL SRAKEIEEQN KQLLEGVEKI ISQAYPEAKG NGIYFVWSQL PSLQGVDEES KILSLRNTIR CLRRDSHKVD NFLKVLRCQI AHQNNC
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant mouse Prolactin was lyophilized from a.2 μm filtered NaHCO3 solution.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
