mouse Prolactin Recombinant
$70.00 – $4,700.00
accession | P06879 |
Source | Optimized DNA sequence encoding Mouse Prolactin mature chain fused to a C terminal polyHis tag was expressed in Insect Cells. |
Molecular weight | Native mouse Prolactin is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 23kDa. Recombinant Prolcatin is a monomeric protein consisting of 200 amino acid residue subunits, and migrates as an approximately 23kDa protein under non-reducing conditions in SDS-PAGE. |
Purity | >95%, as determined by SDS-PAGE and HPLC |
Biological Activity | The activity was determined by the dose-dependent proliferation of Nb2 cells. |
Protein Sequence | MNSQGSAQKA GTLLLLLISN LLFCQNVQPL PICSAGDCQT SLRELFDRVV ILSHYIHTLY TDMFIEFDKQ YVQDREFMVK VINDCPTSSL ATPEDKEQAL KVPPEVLLNL ILSLVQSSSD PLFQLITGVG GIQEAPEYIL SRAKEIEEQN KQLLEGVEKI ISQAYPEAKG NGIYFVWSQL PSLQGVDEES KILSLRNTIR CLRRDSHKVD NFLKVLRCQI AHQNNC |
Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
Presentation | Recombinant mouse Prolactin is presented as concentratedmg/ml,.2 μm filteredmM Tris solution,0.5M NaCl pH.4 containing% trehalose% glycerol. |
Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration of.1 mg/mL. |
Storage | Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
Biological Process | Lactation |
Molecular function | Hormone |