Mouse MIP-3 beta (CCL19) Recombinant

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Mouse MIP-3 beta (CCL19) Recombinant

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SKU: RKO70460 Categories: , Tags: , , ,

accession O70460


Source Optimized DNA sequence encoding Mouse MIP-3 (CCL19) mature chain was expressed in Escherichia Coli.
Molecular weight Mouse CCL19, generated by the proteolytic removal of the signal peptide and propeptide and has a calculated molecular mass of approximately10kDa. Recombinant MIP-3b(CCL19) is a monomeric protein consisting of amino acid residue subunits and migrates as an approximately11 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Purity >98%, as determined by SDS-PAGE and HPLC
Biological Activity Determined by its ability to chemoattract human dendritic cells using a concentration range of.0-50.0 ng/ml.

Protein Sequence MAPRVTPLLA FSLLVLWTFP APTLGGANDA EDCCLSVTQR PIPGNIVKAF RYLLNEDGCR VPAVVFTTLR GYQLCAPPDQ PWVDRIIRRL KKSSAKNKGN STRRSPVS
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant CCL19 was lyophilized from a.2 μm filteredmM PB,100mM NaCl solution pH.5.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor O43927 CXL13_HUMAN
Interactor Q09163
Biological Process Chemotaxis
Biological Process Inflammatory-response
Molecular function Cytokine

Methods

Analysis of chemokine receptors and chemotaxis.

CCL19
  • The migration of MSCs to different concentrations of CXCL12 and CCL19 was evaluated by chemotaxis assay.

Differential activation of MAP kinases in O. tsutsugamushi-infected DCs upon exposure to CCL19.

recombinant chemokine CCL19
  • DCs were stimulated with O. tsutsugamushi , or LPS for 18 h and then further incubated with CCL19 (200 ng/ml) for the indicated times.

Effect of hematopoietic S1P lyase deficiency on T-cell proliferation and migration.

CCL19
  • *P<0.05, **P<0.01, ***P<0.001 (WT versus KO), ###P<0.001 (PBS versus CCL19).

CyaA accelerates cell detachment and migration of TLR-activated DCs.

CCL19
  • Migration of DCs treated with toxins and LPS (for 24 h) towards CCL19 or CCL21 (both 200 ng/ml) in transwell plates was determined by flow cytometry after additional 14 h (MDDCs) or 4 h (BMDCs) of incubation at 37°C.

Mst1 is required for integrin-independent T cell polarization.

100 ng/mL CCL19
  • A) Wt and Mst1 CD4 T cells were stimulated with 100 ng/mL CCL19 in PBS for 30 minutes.