Mouse MIP-1 beta (CCL4) Recombinant

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Mouse MIP-1 beta (CCL4) Recombinant

$70.00$2,700.00


accession P14097


Source Optimized DNA sequence encoding Mouse Macrophage Inflammatory protein-1 beta chain was expressed in Escherichia Coli.
Molecular weight Native Mouse MIP-1 beta is generated by the proteolytic removal of the signal peptide and propeptide. This molecule has a calculated molecular mass of approximately 8 kDa. Recombinant MIP-1 beta is a monomer protein consisting of 69 amino acid residue subunits,and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity Determined by its ability to chemoattract Mouse blood monocytes using a concentration range of.0-10.0 ng/ml.

Protein Sequence MKLCVSALSL LLLVAAFCAP GFSAPMGSDP PTSCCFSYTS RQLHRSFVMD YYETSSLCSK PAVVFLTKRG RQICANPSEP WVTEYMSDLE LN
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant mouse MIP-1 beta was lyophilized from a.2 μm filtered PBS solution.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Biological Process Chemotaxis
Biological Process Inflammatory-response
Molecular function Cytokine

Methods

Binding and in vitro activity of murine 18V4F hybridoma antibody.

CCL4
  • Chemotaxis inhibition by 18V4F hybridoma antibody of CCR5-transfected Ba/F3 cells to 5 ng/mL of CCL3, CCL4, and CCL5.

Binding and in vitro activity of murine 18V4F hybridoma antibody.

CCL4
  • Chemotaxis inhibition by 18V4F hybridoma antibody of CCR5-transfected Ba/F3 cells to 5 ng/mL of CCL3, CCL4, and CCL5.