Mouse MIP-1 beta (CCL4) Recombinant
Categories: CCL chemokinesRecombinant Mouse Cytokines$70.00 – $2,700.00
Description
Accession
P14097
Source
Optimized DNA sequence encoding Mouse Macrophage Inflammatory protein-1 beta chain was expressed in Escherichia Coli.
Molecular weight
Native Mouse MIP-1 beta is generated by the proteolytic removal of the signal peptide and propeptide. This molecule has a calculated molecular mass of approximately 8 kDa. Recombinant MIP-1 beta is a monomer protein consisting of 69 amino acid residue subunits,and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
Determined by its ability to chemoattract Mouse blood monocytes using a concentration range of.0-10.0 ng/ml.
Protein Sequence
MKLCVSALSL LLLVAAFCAP GFSAPMGSDP PTSCCFSYTS RQLHRSFVMD YYETSSLCSK PAVVFLTKRG RQICANPSEP WVTEYMSDLE LN
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant mouse MIP-1 beta was lyophilized from a 0.2 μm filtered PBS solution.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Biological Process
Molecular function
Methods
Binding and in vitro activity of murine 18V4F hybridoma antibody.
- Chemotaxis inhibition by 18V4F hybridoma antibody of CCR5-transfected Ba/F3 cells to 5 ng/mL of CCL3, CCL4, and CCL5.
Binding and in vitro activity of murine 18V4F hybridoma antibody.
- Chemotaxis inhibition by 18V4F hybridoma antibody of CCR5-transfected Ba/F3 cells to 5 ng/mL of CCL3, CCL4, and CCL5.
