Mouse MIF Recombinant

Mouse MIF Recombinant

$70.00$3,500.00

SKU: RKP34884 Category: Tags: , , , , ,

accession P34884


Source Optimized DNA sequence encoding Mouse MIF mature chain was expressed in Escherichia Coli.
Molecular weight Native Mouse MIF, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately15 kDa. Recombinant Mouse MIF is a monomer protein consisting of 115 amino acid residue subunits, migrates as an approximately13 kDa protein under non-reducingand reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity The activity was determined by the ability to bind recombinant human CD74 in functional ELISA.
Protein Sequence
Presentation Recombinant mouse MIF was lyophilized from a 0.2 μm filtered solution PBS pH 7.4
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Biological Process Immunity
Biological Process Inflammatory-response
Molecular function Cytokine
Molecular function Isomerase

Methods

TcAg enhances phospho-ERK independent of MIF, but phospho-38 is related to MIF modulation.

rMIF
  • MIF-/- DCs pulsed with TcAg, rMIF, TcAg+rMIF or PMA as control with or without the p38 inhibitor.