Mouse LIF Recombinant (CHO cell)

////Mouse LIF Recombinant (CHO cell)

Mouse LIF Recombinant (CHO cell)

$160.00$3,500.00

SKU: RCP09056 Category: Tags: , ,

accession P09056


Source Optimized DNA sequence encoding Mouse LIF mature chain was expressed in CHO Cells.
Molecular weight Native Mouse LIF isgenerated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 20kDa. LIF is a monomer protein consisting of 180 amino acid residue subunits, and due to glycosylation migrates as an approximately 35kDa protein under reducing conditions in SDS-PAGE.
Purity >98%, as determined by SDS-PAGE and HPLC.
Biological Activity The ED(50) was determined by the ability to inhibit differentiation of M1 myeloid leukemic cells was found to be in the range of.5 ng/ml.

Protein Sequence MKVLAAGIVP LLLLVLHWKH GAGSPLPITP VNATCAIRHP CHGNLMNQIK NQLAQLNGSA NALFISYYTA QGEPFPNNVE KLCAPNMTDF PSFHGNGTEK TKLVELYRMV AYLSASLTNI TRDQKVLNPT AVSLQVKLNA TIDVMRGLLS NVLCRLCNKY RVGHVDVPPV PDHSDKEAFQ RKKLGCQLLG TYKQVISVVV QAF
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation RecombinantLeukemia Inhibitory Factorwas lyophilized from a.2 μm filtered PBS pH.5.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P42703 LIFR_MOUSE
Molecular function Cytokine
Molecular function Growth-factor