Mouse Leukemia Inhibitory Factor Recombinant
$70.00 – $2,200.00
| accession | P09056 |
| Source | Optimized DNA sequence encoding Mouse LIF mature chain was expressed in Escherichia Coli. |
| Molecular weight | Native Mouse LIF is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 20kDa. LIF is a monomer protein consisting of 181 amino acid residue subunits, and migrates as an approximately 20kDa protein under reducing conditions in SDS-PAGE. |
| Purity | >98%, as determined by SDS-PAGE and HPLC. |
| Biological Activity | The ED(50) was determined by the dose-dependent differentiation of M1 myeloid leukemic cells was found to be in the range of.01 ng/ml. |
| Protein Sequence | MKVLAAGIVP LLLLVLHWKH GAGSPLPITP VNATCAIRHP CHGNLMNQIK NQLAQLNGSA NALFISYYTA QGEPFPNNVE KLCAPNMTDF PSFHGNGTEK TKLVELYRMV AYLSASLTNI TRDQKVLNPT AVSLQVKLNA TIDVMRGLLS NVLCRLCNKY RVGHVDVPPV PDHSDKEAFQ RKKLGCQLLG TYKQVISVVV QAF |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | RecombinantLeukemia Inhibitory Factorwas lyophilized from a.2 μm filtered PBS pH.5. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | P42703 LIFR_MOUSE |
| Molecular function | Cytokine |
| Molecular function | Growth-factor |