Mouse L-Selectin (CD62L) Recombinant

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Mouse L-Selectin (CD62L) Recombinant


accession P18337

Source Optimized DNA sequence encoding extracellular domain of Mouse L-Selectin including a C-terminal IgG1 Fc tag was expressed in HEK293 cells.
Molecular weight Recombinant L-Selectin is a homodimer protein consisting of 540 amino acid residue subunits, due to glycosylation migrates as an approximately 100kDa protein on SDS-PAGE.
Purity >97%, as determined by SDS-PAGE and HPLC
Biological Activity The activity was tested by the ability of immobilized recombinant Mouse L-Selectin to bind Human U937 lymphoma cells
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Mouse L-Selectin (CD62L) is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Storage Recombinant L-Selectin can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Biological Process Cell-adhesion


Flow Cytometric Analysis

  • For flow cytometric analysis of surface markers and intracellular cytokines, enriched C4+ T cells, and single-cell suspensions of lungs were stained with optimal concentrations of the following specific antibodies against surface molecules and intracellular cytokines: C8 α-V450, C4-V500, C11c-FITC, C62L-APC, Gr-1-APC, IFNγ-APC from , , , , and MHCII(I-A/I-E)-eFluor780 from eBioscience, C44-FITC, and IFNγ-PE fromand IL-22-PE from& .
  • Fluorescence intensity was measured by using a FacsCantoII® flow cytometer equipped with a 405 nm, 488 nm and 633 nm laser.
  • Analysis was performed utilizing the FCSExpress4 program (DeNovo™ Software) gated on leukocytes identified by the forward-scatter/side-scatter profile and further characterization as specified in the figures.