Mouse Keratinocyte Growth Factor Recombinant

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Mouse Keratinocyte Growth Factor Recombinant

$70.00 – $4,700.00

SKU: RKP36363 Category: Recombinant Mouse Cytokines Tags: FGF-7, FGF7, HBGF-7, KGF, P36363

Description

Methods (1)


accession	P36363

Source	Optimized DNA sequence encoding Mouse Keratinocyte Growth Factor mature chain was expressed in Escherichia Coli.
Molecular weight	Native Mouse KGF is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately kDa. Recombinant Mouse Keratinocyte Growth Factoris a monomeric protein consisting of amino acid residue subunits. Mouse KGF migrates as an approximately kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Purity	>96%, as determined by SDS-PAGE and HPLC
Biological Activity	The ED(50) was determined by the dose-dependent proliferation ofBAF3 cells was found to be less than10 ng/ml.
Protein Sequence	MHKWILTWIL PTLLYRSCFH IICLVGTISL ACNDMTPEQM ATNVNCSSPE RHTRSYDYME GGDIRVRRLF CRTQWYLRID KRGKVKGTQE MKNNYNIMEI RTVAVGIVAI KGVESEFYLA MNKEGKLYAK KECNEDCNFK ELILENHYNT YASAKWTHNG GEMFVALNQK GIPVRGKKTK KEQKTAHFLP MAIT
Endotoxin	Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation	Recombinant KGF was lyophilized from a.2 μm filteredM NaCl,mM PBsolution pH7.0.
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage	The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Molecular function	Growth-factor
Molecular function	Heparin-binding
Molecular function	Mitogen

Methods


3D cell cultures Dispersed bMECs and hMECs were cultured for 6–7 days, avoiding confluence, in mammary medium [³ cells, were carefully placed at the bottom of wells in 24-well plates and supplemented with 0.5 ml conditioned or fresh mammary medium for 3 days, unless otherwise indicated. Adipose-conditioned medium was collected from 6-day explant cultures [³/ml). When indicated, it was supplemented with hFGF7 and hFGF10 . bMEC-conditioned medium was obtained from 6-day cultures of freshly dissociated bMECs in medium'>mammary medium. Ammonia levels in the conditioned media were monitored during culture using EnzyChrom Ammonia assay kit (ENH3-100, BioAssay Systems, Hayward, CA), according to the manufacturer’s protocol. 3D cultures were visualized after 3 days by light microscopy and mammosphere size was measured using NIS-Elements AR 3.2 software . At least 60 mammospheres were measured in each group. 3D Matrigel cultures were then fixed in Bouin’s solution and processed for histological and histochemical analyses… Dispersed bMECs and hMECs were cultured for 6–7 days, avoiding confluence, in mammary medium [³ cells, were carefully placed at the bottom of wells in 24-well plates and supplemented with 0.5 ml conditioned or fresh mammary medium for 3 days, unless otherwise indicated. Adipose-conditioned medium was collected from 6-day explant cultures [³/ml). When indicated, it was supplemented with hFGF7 and hFGF10 . bMEC-conditioned medium was obtained from 6-day cultures of freshly dissociated bMECs in medium'>mammary medium. Ammonia levels in the conditioned media were monitored during culture using EnzyChrom Ammonia assay kit (ENH3-100, BioAssay Systems, Hayward, CA), according to the manufacturer’s protocol. 3D cultures were visualized after 3 days by light microscopy and mammosphere size was measured using NIS-Elements AR 3.2 software . At least 60 mammospheres were measured in each group. 3D Matrigel cultures were then fixed in Bouin’s solution and processed for histological and histochemical analyses as described above. Read more