Mouse IP-10 Recombinant
Categories: Recombinant Mouse CytokinesCXC chemokines$70.00 – $160.00
Description
Accession
P17515
Source
Optimized DNA sequence encoding Mouse IP-10/CXCL10 mature chain was expressed in Escherichia Coli
Molecular weight
Native Mouse IP10/CXCL10, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 9 kDa. Recombinant IP-10 is a monomer protein consisting of 77 amino acid residue subunits, and migrates as an approximately 9 kDa protein under reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
Determined by its ability to chemoattract IL-2 activated T cells using a concentration range of.1-10.0 ng/ml.
Protein Sequence
MNPSAAVIFC LILLGLSGTQ GIPLARTVRC NCIHIDDGPV RMRAIGKLEI IPASLSCPRV EIIATMKKND EQRCLNPESK TIKNLMKAFS QKRSKRAP
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Interleukin-6 was lyophilized from a 0.2 μm filtered solution in.5% glycine,.5% sucrose,.01% Tween80, mM Glutamic acid, pH.5.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Biological Process
Molecular function
Methods
Secretion of proinflammatory chemokines by TNF-stimulated glomeruli ex vivo.
- Concentrations of CCL2/MCP-1, CCL5/RANTES, and CXCL10/IP-10 were measured by ELISA in culture supernatants of wildtype, Tnfr1−/−, Tnfr2−/−, and Tnfr1,2−/− glomeruli 12 hours after stimulation with 50 ng/ml of TNF.
