Mouse Interleukin-9 Recombinant

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$70.00$4,700.00

SKU: RKP15247 Tags: , , , ,

Description

Accession
P15247
Source
Optimized DNA sequence encoding mouse Interleukin-9 mature chain was expressed in Escherichia Coli.
Molecular weight
Native Mouse Interleukin-9 is generated by the proteolytic removal of the signal peptideand propeptide. This molecule has a calculated molecular mass of approximately 14 kDa. Recombinant Interleukin-9 is a monomer protein consisting of 127 amino acid residue subunits, and migrates as an approximately 14kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the dose-dependent proliferation of human MO7e cells is <.2 ng/ml.

Protein Sequence
MLVTYILASV LLFSSVLGQR CSTTWGIRDT NYLIENLKDD PPSKCSCSGN VTSCLCLSVP TDDCTTPCYR EGLLQLTNAT QKSRLLPVFH RVKRIVEVLK NITCPSFSCE KPCNQTMAGN TLSFLKSLLG TFQKTEMQRQ KSRP
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant mouse IL-9 was lyophilized from a.2 μm filtered PBS solution.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Molecular function

Methods

Mast Cell Cultures

  • CTLMC and MLMC were derived by culturing mouse bone marrow cells from either wild type or MyD88−/− C57BL/6 mice as previously described β1 .
  • All cells were cultured for a minimum of 2 weeks for MLMC and 4 weeks for CTLMC before they were used.
  • Characterization and verification of in vitro-produced CTLMC and MLMC was performed through protease expression as previously described
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