Mouse Interleukin-5 Recombinant

HomeHematopoietins, IL-3 family, Recombinant Mouse Cytokines

Mouse Interleukin-5 Recombinant

$70.00 – $4,700.00

SKU: RKP04401 Categories: Hematopoietins, IL-3 family, Recombinant Mouse Cytokines Tags: BCGF-II, il-5, il5, interleukin-5, P04401, TRF

Description

Methods (7)


accession	P04401

Source	Optimized DNA sequence encoding Mouse Interleukin-5 mature chain was expressed in Insect Cells
Molecular weight	Native Mouse Interleukin-5, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 13 kDa. Recombinant mouse IL-5 is a disulfide-linked homodimeric protein consisting of two 113 amino acid residue subunits,and migrates as an approximately 26 kDa protein under non-reducing conditions and as a 13 kDa protein under reducing conditions in SDS-PAGE.
Purity	>95%, as determined by SDS-PAGE and HPLC
Biological Activity	The ED(50) was determined by the dose-dependent proliferation of TF-1 cells was ≤.2 ng/ml, corresponding to a specific activity of ≥ x units/mg.
Protein Sequence	MRRMLLHLSV LTLSCVWATA MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG
Endotoxin	Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation	Recombinant Interleukin-5 was lyophilized from a.2 μm filtered PBS solution.
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage	The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Molecular function	Cytokine
Molecular function	Growth-factor

Methods


γIMCs are the source of IFN-γ in severe invasive GAS infections. (f) Sorted IMCs were cultured with control medium (Med), G-CSF (50 ng ml−1), M-CSF (10 ng ml−1), GM-CSF (10 ng ml−1), or IL-5 (10 ng ml−1) for 2–4 days, and their absolute numbers were counted on the indicated days.
CD200R and CD200RLc expression on mouse leukocytes. Cells cultured in IL-5 supplemented media were gated as CD11bSiglec F eosinophils in CD11b-Siglec F plot (bottom left).
Effects of DPI on Siglec-F-induced apoptosis of eosinophils. Wild-type mouse bone marrow-derived eosinophils (n = 4) were incubated with or without DPI (20 µM) for 5 min before adding either a control mAb or Siglec-F mAb and then cells were cultured in IL-5 for an additional 18 hr before determining Annexin positivity.
Effects of DPI on Siglec-F-induced apoptosis of eosinophils. Wild-type mouse bone marrow-derived eosinophils (n = 4) were incubated with or without DPI (20 µM) for 5 min before adding either a control mAb or Siglec-F mAb and then cells were cultured in IL-5 for an additional 18 hr before determining Annexin positivity.
Expansion of bmEos ex vivo. Bone marrow cells from BALB/c mice were cultured for 14 days, see Methods and samples taken from day 4 to day 14 in cultures grown in the presence of IL-5.
Generation of bone marrow-derived eosinophils The cells were cultured at 37°C in the presence of 5% CO₂. On day 4, the cells were removed from the flasks by pipetting, centrifuged, and resuspended in an equivalent volume of fresh medium'>BMDE medium containing 10 ng/ml IL-5 . The cells were then transferred to new flasks and cultured at 37°C in the presence of 5% CO₂. On day 8, the cells were again removed from the flasks by pipetting, centrifuged, and resuspended in an equivalent volume of fresh medium'>BMDE medium containing 10 ng/ml IL-5 . The cells were then transferred to new flasks and cultured at 37°C in the presence of 5% CO₂. On days 10, 12, 14, and 16, the cells were removed from the flasks by pipetting, centrifuged, counted, adjusted to a concentration of 10⁶ cells/ml in fresh medium'>BMDE medium containing 10 ng/ml IL-5, and cultured at 37°C in… The cells were cultured at 37°C in the presence of 5% CO₂. On day 4, the cells were removed from the flasks by pipetting, centrifuged, and resuspended in an equivalent volume of fresh medium'>BMDE medium containing 10 ng/ml IL-5 . The cells were then transferred to new flasks and cultured at 37°C in the presence of 5% CO₂. On day 8, the cells were again removed from the flasks by pipetting, centrifuged, and resuspended in an equivalent volume of fresh medium'>BMDE medium containing 10 ng/ml IL-5 . The cells were then transferred to new flasks and cultured at 37°C in the presence of 5% CO₂. On days 10, 12, 14, and 16, the cells were removed from the flasks by pipetting, centrifuged, counted, adjusted to a concentration of 10⁶ cells/ml in fresh medium'>BMDE medium containing 10 ng/ml IL-5, and cultured at 37°C in the presence of 5% CO₂. On day 18, mature eosinophils were removed from the flasks by pipetting, centrifuged, and counted. Read more
Generation of bone marrow-derived eosinophils The cells were cultured at 37°C in the presence of 5% CO₂. On day 4, the cells were removed from the flasks by pipetting, centrifuged, and resuspended in an equivalent volume of fresh medium'>BMDE medium containing 10 ng/ml IL-5 . The cells were then transferred to new flasks and cultured at 37°C in the presence of 5% CO₂. On day 8, the cells were again removed from the flasks by pipetting, centrifuged, and resuspended in an equivalent volume of fresh medium'>BMDE medium containing 10 ng/ml IL-5 . The cells were then transferred to new flasks and cultured at 37°C in the presence of 5% CO₂. On days 10, 12, 14, and 16, the cells were removed from the flasks by pipetting, centrifuged, counted, adjusted to a concentration of 10⁶ cells/ml in fresh medium'>BMDE medium containing 10 ng/ml IL-5, and cultured at 37°C in… The cells were cultured at 37°C in the presence of 5% CO₂. On day 4, the cells were removed from the flasks by pipetting, centrifuged, and resuspended in an equivalent volume of fresh medium'>BMDE medium containing 10 ng/ml IL-5 . The cells were then transferred to new flasks and cultured at 37°C in the presence of 5% CO₂. On day 8, the cells were again removed from the flasks by pipetting, centrifuged, and resuspended in an equivalent volume of fresh medium'>BMDE medium containing 10 ng/ml IL-5 . The cells were then transferred to new flasks and cultured at 37°C in the presence of 5% CO₂. On days 10, 12, 14, and 16, the cells were removed from the flasks by pipetting, centrifuged, counted, adjusted to a concentration of 10⁶ cells/ml in fresh medium'>BMDE medium containing 10 ng/ml IL-5, and cultured at 37°C in the presence of 5% CO₂. On day 18, mature eosinophils were removed from the flasks by pipetting, centrifuged, and counted. Read more