Mouse Interleukin-21 Recombinant
$160.00 – $4,700.00
| accession | Q9ES17 |
| Source | Optimized DNA sequence encoding Human Interleukin-21 mature chain was expressed in Escherichia Coli. |
| Molecular weight | Mouse Interleukin-21 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 15kDa. Recombinant Mouse IL-21 a monomeric protein consisting of 132 amino acid residue subunits, and migrates as an approximately 15kDa protein under non-reducing and reducing conditions in SDS-PAGE. |
| Purity | >97%, as determined by SDS-PAGE and HPLC |
| Protein Sequence | MERTLVCLVV IFLGTVAHKS SPQGPDRLLI RLRHLIDIVE QLKIYENDLD PELLSAPQDV KGHCEHAAFA CFQKAKLKPS NPGNNKTFII DLVAQLRRRL PARRGGKKQK HIAKCPSCDS YEKRTPKEFL ERLKWLLQKM IHQHLS |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Recombinant Interleukin-21 was lyophilized from a.2 μm filtered PBS solution pH7.5. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Molecular function | Cytokine |
Methods
Exhausted CD8 T cells do not upregulate CD25 expression following IL-12, IL-18, and/or IL-21 exposure.
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Exhausted CD8 T cells do not upregulate CD25 expression following IL-12, IL-18, and/or IL-21 exposure.
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TGF-β enhances the effect of IL-21 on the development of IL-21-producing CD4+ T cells.
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The CD4+ T cell computational model predicts modulation of differentiation and maintenance by IL-21 in silico.
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The effect of IL-21 and CD40L exposure on MEC1 and MEC2 cells.
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