Mouse Interleukin-11 Recombinant
$70.00 – $160.00
| accession | P47873 |
| Source | Optimized DNA sequence encoding Mouse Interleukin-11 mature chain was expressed in Escherichia Coli. |
| Molecular weight | Native Mouse IL-11, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 19 kDa. Recombinant IL-11 is a monomer protein consisting of 180 amino acid residue subunits, and migrates as an approximately 19 kDa protein under reducing conditions in SDS-PAGE. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | The ED(50) was determined by the dose-dependent stimulation of the proliferation ofmurine T11 cells is ≤.0 ng/ml, corresponding to a specific activity of ≥1 xunits/mg. |
| Protein Sequence | MNCVCRLVLV VLSLWPDRVV APGPPAGSPR VSSDPRADLD SAVLLTRSLL ADTRQLAAQM RDKFPADGDH SLDSLPTLAM SAGTLGSLQL PGVLTRLRVD LMSYLRHVQW LRRAGGPSLK TLEPELGALQ ARLERLLRRL QLLMSRLALP QAAPDQPVIP LGPPASAWGS IRAAHAILGG LHLTLDWAVR GLLLLKTRL |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Recombinant mouse IL-11 was lyophilized from a 0.2 μm filtered PBS solution. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | Q64385 I11RA_MOUSE |
| Interactor | Q00560 IL6RB_MOUSE |
| Interactor | Q64385 I11RA_MOUSE |
| Molecular function | Cytokine |
| Molecular function | Growth-factor |
Methods
Effect of IL-11 on invasion of JEG-3 and HTR-8/SVneo cells.
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Lentiviral transduction of bone marrow cells
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