Mouse Interleukin-11 Recombinant

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Mouse Interleukin-11 Recombinant

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accession P47873


Source Optimized DNA sequence encoding Mouse Interleukin-11 mature chain was expressed in Escherichia Coli.
Molecular weight Native Mouse IL-11, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 19 kDa. Recombinant IL-11 is a monomer protein consisting of 180 amino acid residue subunits, and migrates as an approximately 19 kDa protein under reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity The ED(50) was determined by the dose-dependent stimulation of the proliferation ofmurine T11 cells is ≤.0 ng/ml, corresponding to a specific activity of ≥1 xunits/mg.
Protein Sequence MNCVCRLVLV VLSLWPDRVV APGPPAGSPR VSSDPRADLD SAVLLTRSLL ADTRQLAAQM RDKFPADGDH SLDSLPTLAM SAGTLGSLQL PGVLTRLRVD LMSYLRHVQW LRRAGGPSLK TLEPELGALQ ARLERLLRRL QLLMSRLALP QAAPDQPVIP LGPPASAWGS IRAAHAILGG LHLTLDWAVR GLLLLKTRL
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant mouse IL-11 was lyophilized from a.2 μm filtered PBS solution.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor Q64385 I11RA_MOUSE
Interactor Q00560 IL6RB_MOUSE
Interactor Q64385 I11RA_MOUSE
Molecular function Cytokine
Molecular function Growth-factor

Methods

Effect of IL-11 on invasion of JEG-3 and HTR-8/SVneo cells.

IL-11
  • Data is expressed as fold change in invasion following IL-11 (200 ng/ml) treatment as compared to untreated JEG-3 cells as control.

Lentiviral transduction of bone marrow cells

  • Freshly isolated bone marrow (BM) cells were plated in Iscove's Modified Dulbecco's Medium (IMDM) (31980, , ) containing 5% fetal bovine serum (FBS), 1% penicillin/streptomycin, 200 mM glutamine, 1% non-essential amino acids, 1% sodium pyruvate, 50 μM 2-mercaptoethanol, stem cell factor (SCF, 10 ng/ml, , ), Flt3-L (10 ng/ml), IL-11 (10 ng/ml), thrombopoietin (TPO, 10 ng/ml), IL-6 (10 ng/ml), and IL-3 (10 ng/ml).
  • Bone marrow cells were spin-infected with 8 μg/ml polyprene and lentivirus (MOI of 0.1–0.5), at 2,500 rpm for 90 min.
  • BM cells were then incubated for 3 hours at 37°C, counted and injected into NOD.SCID mice.