Mouse Interferon-gamma Recombinant

HomeMouse reagentsRecombinant Mouse Cytokines

Mouse Interferon-gamma Recombinant

$70.00 – $1,000.00

SKU: RKP01580 Category: Recombinant Mouse Cytokines Tags: ifn-gamma, ifng, interferon gamma, P01580

Description

Methods (50)


accession	P01580

Source	Optimized DNA sequence encoding Mouse Interferon gamma mature chain was expressed in Escherichia Coli.
Molecular weight	Native Mouse IFN-g, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 16kDa. Recombinant Interferon gamma is a monomer protein consisting of 134 amino acid residue subunits, and migrates as an approximately 16kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity	>95%, as determined by SDS-PAGE and HPLC
Biological Activity	The ED(50) was determined by the cytopathic inhibition assay with murine L929 cells challenged with EMC virus was found to be.1 ng/ml
Protein Sequence	MNATHCILAL QLFLMAVSGC YCHGTVIESL ESLNNYFNSS GIDVEEKSLF LDIWRNWQKD GDMKILQSQI ISFYLRLFEV LKDNQAISNN ISVIESHLIT TFFSNSKAKK DAFMSIAKFE VNNPQVQRQA FNELIRVVHQ LLPESSLRKR KRSRC
Endotoxin	Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation	Recombinant mouse IFN-g was lyophilized from a.2 μm filtered PBS solution.
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage	The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Biological Process	Antiviral-defense
Biological Process	Growth-regulation
Molecular function	Cytokine

Methods


ΤCRαβ expression by subpopulations of human and murine monocytes/macrophages. (Bottom) LSC of naïve and IL-4 (10 ng/ml) or IFNγ (1000 U/ml) stimulated monocyte-derived macrophages, respectively, cultured for 6 days.
Cardiac adherent proliferating cells reduce Coxsackievirus B3 viral progeny release in a nitric oxide- and interleukin-10-dependent manner and require interferon-γ priming. HeLa cells were incubated for 30 minutes with 1 ml of diluted supernatant of CVB3-infected HL-1 cells or HL-1 cells co-cultured with CAPs, in the presence or absence of L-NAME, anti-human IL-10 antibody, or anti-murine IFN-γ antibody, or with diluted LV homogenates from CVB3 or CVB3+CAPs mice.
Cardiac adherent proliferating cells reduce Coxsackievirus B3 viral progeny release in a nitric oxide- and interleukin-10-dependent manner and require interferon-γ priming. HeLa cells were incubated for 30 minutes with 1 ml of diluted supernatant of CVB3-infected HL-1 cells or HL-1 cells co-cultured with CAPs, in the presence or absence of L-NAME, anti-human IL-10 antibody, or anti-murine IFN-γ antibody, or with diluted LV homogenates from CVB3 or CVB3+CAPs mice.
Cytokine measurements The cytokine concentration in the BAL fluid was quantified by ELISA kits specific for IL-5 and IL-10 and for IFN-γ (INC.). The values are expressed as picograms per milliliter deduced from standards, run in parallel with the recombinant cytokines. The limit of detection values were 10 pg/mL for IL-5 and IL-10 and 16 pg/mL for IFN-γ.
Selection of a β-TC3 cell population resistant to cytokine-mediated cytotoxicity. Cytotoxicity assay in β-TC3 (left) and β-TC3R (right) after cytokine treatment (100 IU/ml IL-1β, 100 IU/ml IFN-γ or their combination, for 72 hours).
Cytokine Assays ELISAs were also performed to assess levels of interleukin (IL)-2, IL-4, IL-10, and interferon (IFN)-γ in the supernatant of the MLCs on day 4. The capture monoclonal antibody (mAb) (JES5-2A5), detection mAb (JES5-16E3), and recombinant standard for IL-10 were from . The capture and detection mAbs for IL-2 (JES6-1A12 and JES6-5H4, respectively), IL-4 (BVD-1D11 and BVD-24G2), and IFN-γ (R4-6A2 and XMG1.2) were from . Recombinant standards for IL-2, IL-4, and IFN-γ were from .
Cell culture Phoenix cells were cultured and maintained in Dulbecco's Modified Eagle's Medium (DMEM supplemented with glutamine, 4.5 g/l glucose), 10% (v/v) fetal bovine serum (FBS) and 1% (v/v) penicillin/streptomycin (PAA) at 37 °C and 5% CO₂. MuSK and MuSK myoblasts isolated from embryos carrying a temperature-sensitive SV40 T antigen were propagated at 33 °C and 5% CO₂ on 0.2% gelatin-coated dishes containing growth medium: DMEM containing glutamine, 4.5 g/l glucose enriched with 10% (v/v) FBS, 10% (v/v) horse serum (HS), 0.25% chick embryo extract (CEE), 20 U/ml recombinant murine interferon-γ (IFN-γ) and 1% (v/v) penicillin/streptomycin (MuSK muscle cells was performed according to Herbst and Burden (MuSK myoblasts in the presence of 2 μg/ml polybrene . After 2 h the virus-containing medium was replaced with fresh growth media. 24 h post-infection, muscle cells were split and maintained in growth medium with puromycin (2 ng/ml). Clones of cells were… Phoenix cells were cultured and maintained in Dulbecco's Modified Eagle's Medium (DMEM supplemented with glutamine, 4.5 g/l glucose), 10% (v/v) fetal bovine serum (FBS) and 1% (v/v) penicillin/streptomycin (PAA) at 37 °C and 5% CO₂. MuSK and MuSK myoblasts isolated from embryos carrying a temperature-sensitive SV40 T antigen were propagated at 33 °C and 5% CO₂ on 0.2% gelatin-coated dishes containing growth medium: DMEM containing glutamine, 4.5 g/l glucose enriched with 10% (v/v) FBS, 10% (v/v) horse serum (HS), 0.25% chick embryo extract (CEE), 20 U/ml recombinant murine interferon-γ (IFN-γ) and 1% (v/v) penicillin/streptomycin (MuSK muscle cells was performed according to Herbst and Burden (MuSK myoblasts in the presence of 2 μg/ml polybrene . After 2 h the virus-containing medium was replaced with fresh growth media. 24 h post-infection, muscle cells were split and maintained in growth medium with puromycin (2 ng/ml). Clones of cells were isolated, expanded and assayed for their ability to differentiate into multinucleated myotubes by removing IFN-γ and CEE from the medium and increasing the temperature to 37 °C. Read more
MHC-II surface expression on KI and KO antigen presenting cells is similar to WT Thioglycolate elicited peritoneal macrophages were stimulated in vitro 24 h with 500 U/ml IFN-γ.
Mechanisms of B cell developmental defects in adolescent Sf mice. FACS-purified populations of BM-derived B220+c-kit+ Pro/Pre-B-I cells from WT mice were cultured with IL-7, either alone or in the presence of titrating amounts of IL-17A or IFN-γ, as indicated.
Effect of omentum cells on ex vivo T cell proliferation. (e) iNOS expression was determined in total omentum cells, CD45+ omentum cells, or CD45− omentum cells upon IFNγ stimulation for 24 hrs by western blotting.