Mouse Insulin Like Growth Factor-1 Recombinant

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Mouse Insulin Like Growth Factor-1 Recombinant

$70.00$1,350.00


accession P05017


Source Optimized DNA sequence encoding mouse Insulin Like Growth Factor-1 mature chain was expressed in Escherichia Coli.
Molecular weight Native Mouse IGF-1, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately kDa. Recombinant IGF1 is a disulfide-linked homodimeric protein consisting of amino acid residue subunits, and migrates as an approximately kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity The ED(50) was determined by the dose-dependent stimulation of the proliferation of monkeyMBr-5 cells was found to be in the range of.0-40.0 ng/ml.

Protein Sequence MGKISSLPTQ LFKICLCDFL KIKIHIMSSS HLFYLALCLL TFTSSTTAGP ETLCGAELVD ALQFVCGPRG FYFNKPTGYG SSIRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPTKAA RS IRAQRHTDMP KTQKEVHLKN TSRGSAGNKT YRM
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant mouse IGF-1 was lyophilized from a.2 μm filtered solution in.5% glycine,.5% sucrose,.01% Tween80, mM Glutamic acid, pH.5.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Molecular function Growth-factor

Methods

LPLI regulates different osteogenic differentiation markers.

IGF1
  • IGF1.

In vitro differentiation of Nes-GFP+ cells

  • LC differentiation For LC lineage differentiation, the Nes-GFP+ cells were replated in fresh differentiation-inducing medium containing phenol red-free DMEM/F12, 2% FCS, 10 ng/ml PDGF-BB , 1 ng/ml LH , 1 nM thyroid hormone , 70 ng/ml insulin-like growth factor 1 (IGF1), and ITS supplement , and they were incubated for 7 days, as previously described.
  • Differentiation was subsequently confirmed by RT-PCR and immunostaining for LC lineage markers (antibodies shown in