Mouse Growth Hormone Recombinant

///Mouse Growth Hormone Recombinant

Mouse Growth Hormone Recombinant

$70.00$2,400.00


accession P06880


Source Optimized DNA sequence encoding Mouse Growth Hormonemature chain was expressed in Escherichia Coli.
Molecular weight Native MouseGrowth Hormone isgenerated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately kDa. Recombinant GH is a disulfide-linked homodimeric protein consisting of191 amino acid residue subunits, andmigrates as an approximately kDa protein under non-reducingand reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity The ED(50) was determined by the dose-dependentcell proliferation assay using the rat lymphoma, Nb2-11, and was found to be in the range of0.1 ng/ml.

Protein Sequence MATDSRTSWL LTVSLLCLLW PQEASAFPAM PLSSLFSNAV LRAQHLHQLA ADTYKEFERA YIPEGQRYSI QNAQAAFCFS ETIPAPTGKE EAQQRTDMEL LRFSLLLIQS WLGPVQFLSR IFTNSLMFGT SDRVYEKLKD LEEGIQALMQ ELEDGSPRVG QILKQTYDKF DANMRSDDAL LKNYGLLSCF KKDLHKAETY LRVMKCRRFV ESSCAF
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant Mouse Growth Hormone was lyophilized from.2 μm filtered100mM NaCl,mM PB, pH.4.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Molecular function Hormone