Mouse GRO-gamma (CXCL3) Recombinant
$70.00 – $2,700.00
| accession | Q6W5C0 |
| Source | Optimized DNA sequence encoding mouse GRO-gamma mature chain was expressed in Escherichia Coli |
| Molecular weight | Native mouse GRO-gamma is generated by the proteolytic removal of the signal peptide and propeptide. The molecule has a calculated molecular mass of approximately 8 kDa. Recombinant GRO-gamma is a disulfide-linked homodimeric protein consisting of 70 amino acid residue subunits, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | Determined by its ability to chemoattract 293 transfected CXCR2 cells using a concentration range of 10-100 ng/ml. |
| Protein Sequence | |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | Recombinant mouse GRO-gamma/CXCL3 was lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in 40mM NaCl, 10mM PB, pH 7.0. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers |
| Storage | The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Biological Process | Chemotaxis |
| Biological Process | Inflammatory-response |
| Molecular function | Cytokine |