Mouse GRO-beta (CXCL2) Recombinant

///Mouse GRO-beta (CXCL2) Recombinant

Mouse GRO-beta (CXCL2) Recombinant

$70.00$2,700.00

SKU: RKP10889 Category: Tags: , , , ,

accession P10889


Source Optimized DNA sequence encoding mouse GRO-beta CXCL2 mature chain was expressed in Escherichia Coli.
Molecular weight Native mouse GRO-beta is generated by the proteolytic removal of the signal peptide and propeptide. The molecule has a calculated molecular mass of approximately kDa. Recombinant GRO-beta is a disulfide-linked homodimeric protein consisting of amino acid residue subunits, and migrates as an approximately kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity Determined by its ability to chemoattract transfected CXCR2 cells using a concentration range of-100 ng/ml.

Protein Sequence MAPPTCRLLS AALVLLLLLA TNHQATGAVV ASELRCQCLK TLPRVDFKNI QSLSVTPPGP HCAQTEVIAT LKGGQKVCLD PEAPLVQKII QKILNKGKAN
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant GRO-beta/CXCL2 was lyophilized from a.2μm filtered concentrated (1mg/ml) solution inmM Tris, pH.0.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor Q2F862
Biological Process Chemotaxis
Biological Process Inflammatory-response
Molecular function Cytokine

Methods

Rab27a promotes migration of neutrophils in vivo and in vitro.

recombinant murine MIP-2
  • Leukocytes recruited to the bronchoalveolar space of WT and Rab27a KO mice treated intranasally with MIP-2; cells were stained with antibody against Ly6G and analysed by flow cytometry.

Rab27a promotes migration of neutrophils in vivo and in vitro.

towards recombinant murine MIP-2
  • Leukocytes recruited to the bronchoalveolar space of WT and Rab27a KO mice treated intranasally with MIP-2; cells were stained with antibody against Ly6G and analysed by flow cytometry.