Mouse GRO-alpha (CXCL1)Recombinant
$70.00 – $2,700.00
accession | P12850 |
Source | Optimized DNA sequence encoding mouse GRO alpha mature chain was expressed in Escherichia Coli. |
Molecular weight | Native mouse CXCL1/GRO-alpha is generated by the proteolytic removal of the signal peptide and propeptide. The molecule has a calculated molecular mass of approximately 8 kDa. Recombinant mouse CXCL1/GRO-a is a monomer protein consisting of 72 amino acid residue subunits, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE. |
Purity | >97%, as determined by SDS-PAGE and HPLC |
Biological Activity | Determined by theability to chemoattract human neutrophils using a concentration range of.0-50.0 ng/ml. |
Protein Sequence | MIPATRSLLC AALLLLATSR LATGAPIANE LRCQCLQTMA GIHLKNIQSL KVLPSGPHCT QTEVIATLKN GREACLDPEA PLVQKIVQKM LKGVPK |
Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
Presentation | Recombinant mouse GRO-alpha/CXCL1was lyophilized from a.2μm filtered PBS pH.4. |
Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
Biological Process | Inflammatory-response |
Molecular function | Cytokine |
Molecular function | Growth-factor |