Mouse GM-CSF Recombinant (CHO cell)
$70.00 – $2,900.00
| accession | P04141 |
| Source | Optimized DNA sequence encoding Mouse Granulocyte Macrophage-Colony Stimulating Factor mature chain was expressed in CHO Cells. |
| Molecular weight | Native Mouse Granulocyte Macrophage-Colony Stimulating Factor is generated by the proteolytic removal of the signal peptide and propeptide,this molecule has a calculated mass of 15 kDa. Recombinant GM-CSFis a monomer protein, consisting of 125 amino acidsand due to glycosylation migrates as an approximately 25 kDa protein under reducing conditions. |
| Purity | >96%, as determined by SDS-PAGE and HPLC |
| Biological Activity | The ED50 as determined by the dose-dependent stimulation of the proliferation of FDC-P1 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg. |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | Mouse GM-CSFwas lyophilized from a 0.2 μm filtered solution in PBS, pH 7.0. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | P15509 CSF2R_HUMAN |
| Interactor | Q99062 CSF3R_HUMAN |
| Interactor | P32927 IL3RB_HUMAN |
| Molecular function | Cytokine |
| Molecular function | Growth-factor |