////Mouse GM-CSF Recombinant (CHO cell)

Mouse GM-CSF Recombinant (CHO cell)

Category:

$70.00$2,900.00

SKU: RCP01587 Tags: , , , , , ,

Description

Accession
P04141
Source
Optimized DNA sequence encoding Mouse Granulocyte Macrophage-Colony Stimulating Factor mature chain was expressed in CHO Cells.
Molecular weight
Native Mouse Granulocyte Macrophage-Colony Stimulating Factor is generated by the proteolytic removal of the signal peptide and propeptide,this molecule has a calculated mass of 15 kDa. Recombinant GM-CSFis a monomer protein, consisting of 125 amino acidsand due to glycosylation migrates as an approximately 25 kDa protein under reducing conditions.
Purity
>96%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED50 as determined by the dose-dependent stimulation of the proliferation of FDC-P1 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Mouse GM-CSFwas lyophilized from a 0.2 μm filtered solution in PBS, pH 7.0.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Molecular function
Molecular function
Stay in the loop
For new product information and regular updates on our product range please fill your details
Loading