Mouse Exodus-2 Recombinant

Mouse Exodus-2 Recombinant

$70.00$2,700.00


accession P84444


Source Optimized DNA sequence encoding Mouse Exodus-2 (CCL21) mature chain was expressed in Escherichia Coli.
Molecular weight Native mouse CCL21/Exodus-2 is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated molecular mass of approximately 12 kDa. Recombinant mouse Exodus-2 is a monomer protein consisting of 111 amino acid residue subunits, and migrates as an approximately 12 kDa protein under reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity Determined by its ability to chemoattract total lymphocyte population using a concentration range of.0-100.0 ng/ml.

Protein Sequence MAQMMTLSLL SLVLALCIPW TQGSDGGGQD CCLKYSQKKI PYSIVRGYRK QEPSLGCPIP AILFSPRKHS KPELCANPEE GWVQNLMRRL DQPPAPGKQS PGCRKNRGTS KSGKKGKGSK GCKRTEQTQP SRG
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant mouse Exodus-2 was lyophilized from a.2 μm filtered PBS solution.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Biological Process Chemotaxis
Biological Process Inflammatory-response
Molecular function Cytokine

Methods

BMMCs promote the maturation and chemotactic activity of BMDCs via direct cell interaction.

CCL21
  • The expression levels of CD40, CD80, CD86 and CCR7 on BMDCs cultured with or without BMMCs for 24 h. Mobility of BMMCs to CCL21.

Migration Assays

  • Stromal cells were prepared from the peripheral lymph nodes as previously described β cells expressing WT or mutant OCK2 were placed on a monolayer of stromal cells in the presence or absence of CCL21 (100 nM& , , ).
  • After 4 hours of incubation, phase-contrast images were obtained every 30 seconds for 20 minutes at 37°C on an IX-81 inverted microscope .
  • Migration of individual cells was tracked using the MetaMorph imaging software , and the migration speed was calculated by dividing the total path length by the total assay time.

Non-competitive CCR7-mediated chemotaxis assay of CD11c+ lung cells

  • The chemotaxis assay was performed as previously described 5 CD11c+ lung cells in a total volume of 100 µl chemotaxis medium (RPMI-1640, 0.5% BSA) was added to the upper chambers of 24-well transwell plates with 5-µm-pore-size polycarbonate inserts .
  • 600 µl of chemotaxis medium containing either CCL19 (100 ng/ml, Shenandoah Biotechnology, Warwick, PA), CCL21 (100 ng/ml, , ) or CCL28 (100 ng/ml) were added to the lower chambers.
  • After 5 hours of incubation at 37°C, migrated cells from the lower chambers were collected, stained with anti-CD11c and anti-MHC-II , and enumerated with Trucount beads by flow cytometry ( FACS ).

CyaA accelerates cell detachment and migration of TLR-activated DCs.

CCL21
  • Migration of DCs treated with toxins and LPS (for 24 h) towards CCL19 or CCL21 (both 200 ng/ml) in transwell plates was determined by flow cytometry after additional 14 h (MDDCs) or 4 h (BMDCs) of incubation at 37°C.

In vitro and in vivo expression and biological activity of murine CCL21.

murine CCL21
  • (a) Western blot analysis of murine CCL21 expression after infection of Hela cells.